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A Photodeactivatable Antagonist for Controlling CREB-Dependent Gene Expression.
Imoto, Takuma; Minoshima, Masafumi; Yokoyama, Tatsushi; Emery, Ben P; Bull, Steven D; Bito, Haruhiko; Kikuchi, Kazuya.
Affiliation
  • Imoto T; Division of Advanced Science and Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan.
  • Minoshima M; Division of Advanced Science and Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan.
  • Yokoyama T; Department of Neurochemistry, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
  • Emery BP; Department of Chemistry, University of Bath, Bath BA2 7AY, United Kingdom.
  • Bull SD; Department of Chemistry, University of Bath, Bath BA2 7AY, United Kingdom.
  • Bito H; Department of Neurochemistry, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
  • Kikuchi K; Division of Advanced Science and Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan.
ACS Cent Sci ; 6(10): 1813-1818, 2020 Oct 28.
Article in En | MEDLINE | ID: mdl-33145417
ABSTRACT
A novel photodeactivation strategy for controlling gene expression has been developed based on light-induced activation of cAMP response element binding protein (CREB). Light-induced cleavage of the photoresponsive protecting group of an antagonist of CREB binding protein (CBP) results in photocleaved products with weak binding affinity for CBP. This photodissociation reaction enables protein-protein interactions between CBP and CREB that trigger the formation of a multiprotein transcription complex to turn gene expression "on". This enables irradiation of antagonist-treated HEK293T cells to be used to trigger temporal recovery of CREB-dependent transcriptional activity and endogenous gene expression under photolytic control.

Full text: 1 Collection: 01-internacional Database: MEDLINE Language: En Journal: ACS Cent Sci Year: 2020 Document type: Article Affiliation country: Japan

Full text: 1 Collection: 01-internacional Database: MEDLINE Language: En Journal: ACS Cent Sci Year: 2020 Document type: Article Affiliation country: Japan