A Photodeactivatable Antagonist for Controlling CREB-Dependent Gene Expression.
ACS Cent Sci
; 6(10): 1813-1818, 2020 Oct 28.
Article
in En
| MEDLINE
| ID: mdl-33145417
ABSTRACT
A novel photodeactivation strategy for controlling gene expression has been developed based on light-induced activation of cAMP response element binding protein (CREB). Light-induced cleavage of the photoresponsive protecting group of an antagonist of CREB binding protein (CBP) results in photocleaved products with weak binding affinity for CBP. This photodissociation reaction enables protein-protein interactions between CBP and CREB that trigger the formation of a multiprotein transcription complex to turn gene expression "on". This enables irradiation of antagonist-treated HEK293T cells to be used to trigger temporal recovery of CREB-dependent transcriptional activity and endogenous gene expression under photolytic control.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Language:
En
Journal:
ACS Cent Sci
Year:
2020
Document type:
Article
Affiliation country:
Japan