Your browser doesn't support javascript.
loading
Persistence of Markers of Chloroquine Resistance in Plasmodium falciparum in Bangladesh.
Johora, Fatema Tuj; Elahi, Rubayet; Nima, Maisha Khair; Hossain, Mohammad Sharif; Rashid, Humaira; Kibria, Mohammad Golam; Mohon, Abu Naser; Khan, Wasif A; Haque, Rashidul; Alam, Mohammad Shafiul.
Affiliation
  • Johora FT; 1Infectious Diseases Division, International Centre for Diarrhoeal Diseases Research, Bangladesh (icddr,b), Dhaka, Bangladesh.
  • Elahi R; 1Infectious Diseases Division, International Centre for Diarrhoeal Diseases Research, Bangladesh (icddr,b), Dhaka, Bangladesh.
  • Nima MK; 2Department of Molecular Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland.
  • Hossain MS; 1Infectious Diseases Division, International Centre for Diarrhoeal Diseases Research, Bangladesh (icddr,b), Dhaka, Bangladesh.
  • Rashid H; 3Department of Biological Sciences, Boler-Parseghian Center for Rare and Neglected Diseases, University of Notre Dame, Notre Dame, Indiana.
  • Kibria MG; 1Infectious Diseases Division, International Centre for Diarrhoeal Diseases Research, Bangladesh (icddr,b), Dhaka, Bangladesh.
  • Mohon AN; 1Infectious Diseases Division, International Centre for Diarrhoeal Diseases Research, Bangladesh (icddr,b), Dhaka, Bangladesh.
  • Khan WA; 1Infectious Diseases Division, International Centre for Diarrhoeal Diseases Research, Bangladesh (icddr,b), Dhaka, Bangladesh.
  • Haque R; 1Infectious Diseases Division, International Centre for Diarrhoeal Diseases Research, Bangladesh (icddr,b), Dhaka, Bangladesh.
  • Alam MS; 4Department of Microbiology, Immunology and Infectious Disease, University of Calgary, Alberta, Canada.
Am J Trop Med Hyg ; 104(1): 276-282, 2021 01.
Article in En | MEDLINE | ID: mdl-33146120
ABSTRACT
The control of malaria, in terms of drug resistance, remains a significant global challenge, with Bangladesh, a malaria-endemic country, being no exception. The aim of this study was to explore antimalarial resistance in Bangladesh by molecular analysis of Plasmodium falciparum chloroquine resistance transporter (pfcrt) and P. falciparum multidrug resistance transporter 1 (pfmdr1) genetic markers of P. falciparum. Samples were obtained from uncomplicated malaria patients between 2009 and 2014 from six malaria-endemic districts. Based on parasite transmission intensity, the endemic districts were divided into high-transmission (Chittagong Hill Tracts [CHT]) and low-transmission (non-CHT) regions. Falciparum malaria-positive isolates were genotyped for K76T of the pfcrt gene, and N86Y and Y184F of the pfmdr1 gene in total, 262 P. falciparum clinical isolates were analyzed. In CHT areas, the prevalence of polymorphisms was 70.6% for 76T, 14.4% for 86Y, and 7.8% for 184F. In non-CHT areas, 76T and 86Y mutations were found in 78.0% and 19.5% of the samples, respectively, whereas no 184F mutations were observed. We compared our data with previous similar molecular observations, which shows a significant decrease in pfcrt 76T mutation prevalence. No pfmdr1 amplification was observed in any of the samples suggesting an unaltered susceptibility to amino alcohol drugs such as mefloquine and lumefantrine. This study provides an updated assessment of the current status of pfcrt and pfmdr1 gene mutations in Bangladesh, and suggests there is persistent high prevalence of markers of resistance to aminoquinoline drugs.
Subject(s)

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Plasmodium falciparum / Genetic Markers / Chloroquine / Malaria, Falciparum / Antimalarials Type of study: Risk_factors_studies Limits: Humans Country/Region as subject: Asia Language: En Journal: Am J Trop Med Hyg Year: 2021 Document type: Article Affiliation country: Bangladesh

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Plasmodium falciparum / Genetic Markers / Chloroquine / Malaria, Falciparum / Antimalarials Type of study: Risk_factors_studies Limits: Humans Country/Region as subject: Asia Language: En Journal: Am J Trop Med Hyg Year: 2021 Document type: Article Affiliation country: Bangladesh