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Evaluation of the diagnostic accuracy of lateral flow devices as a tool to diagnose rabies in post-mortem animals.
Kimitsuki, Kazunori; Saito, Nobuo; Yamada, Kentaro; Park, Chun-Ho; Inoue, Satoshi; Suzuki, Motoi; Saito-Obata, Mariko; Kamiya, Yasuhiko; Manalo, Daria L; Demetria, Catalino S; Mananggit, Milagros R; Quiambao, Beatriz P; Nishizono, Akira.
Affiliation
  • Kimitsuki K; Department of Microbiology, Faculty of Medicine, Oita University, Yufu, Oita, Japan.
  • Saito N; Department of Microbiology, Faculty of Medicine, Oita University, Yufu, Oita, Japan.
  • Yamada K; Laboratory of Veterinary Public Health, Department of Veterinary Medical Science, Faculty of Agriculture, University of Miyazaki, Miyazaki, Miyazaki, Japan.
  • Park CH; Department of Veterinary Pathology, School of Veterinary Medicine, Kitasato University, Towada, Aomori, Japan.
  • Inoue S; National Institute of Infectious Disease, Tokyo, Japan.
  • Suzuki M; National Institute of Infectious Disease, Tokyo, Japan.
  • Saito-Obata M; Tohoku University Graduate School of Medicine, Sendai, Miyagi, Japan.
  • Kamiya Y; School of Tropical Medicine & Global Health, Nagasaki University, Nagasaki, Nagasaki, Japan.
  • Manalo DL; Research Institute for Tropical Medicine, Muntinlupa City, Metro Manila, Philippines.
  • Demetria CS; Research Institute for Tropical Medicine, Muntinlupa City, Metro Manila, Philippines.
  • Mananggit MR; Regional Animal Disease Diagnostic Laboratory, Department of Agriculture Field Office III, San Fernando, Pampanga, Philippines.
  • Quiambao BP; Research Institute for Tropical Medicine, Muntinlupa City, Metro Manila, Philippines.
  • Nishizono A; Department of Microbiology, Faculty of Medicine, Oita University, Yufu, Oita, Japan.
PLoS Negl Trop Dis ; 14(11): e0008844, 2020 11.
Article in En | MEDLINE | ID: mdl-33151941
Implementation of lateral flow devices (LFDs) for rabies antigen detection is expected to improve surveillance through the efficient detection of rabid animals in resource-limited settings; however, the use of LFDs for diagnosis remains controversial because some commercially available kits show low sensitivity. Therefore, we compared the diagnostic efficacy of three LFDs (ADTEC, Bionote, and Elabscience kits) paralleled with the direct fluorescent antibody test (dFAT) using fresh samples and investigated the diagnostic accuracies. To do so, we evaluated rabies-suspected samples submitted to the Regional Animal Disease Diagnostic Laboratory III, Philippines. Furthermore, we conducted real-time RT-PCR and sequencing to measure the accuracy of field laboratory diagnosis. The total number of animals submitted during this study period was 184 cases, including negative control samples. Of these, 53.9% (84 cases) were positive in the dFAT. Dogs were the most common rabies-suspected animal (n = 135). The sensitivities of the ADTEC and Bionote kits were 0.88 (74 cases) and 0.95 (80 cases), respectively. The specificity of both kits was 1.00 (100 cases). Furthermore, the sensitivity and specificity of the ADTEC kit after directly homogenizing the samples in assay buffer without dilution in phosphate-buffered saline (ADTEC kit DM) were 0.94 (79 cases) and 1.00 (100 cases), respectively. By contrast, there were no positive results using the Elabscience kit among all dFAT-positive samples. The sensitivity and specificity of LFDs make these tests highly feasible if properly used. Therefore, LFD tests can be used to strengthen the surveillance of rabies-infected animals in endemic and resource-limited settings.
Subject(s)

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Rabies / Rabies virus / Reagent Kits, Diagnostic Type of study: Diagnostic_studies / Evaluation_studies Limits: Animals Language: En Journal: PLoS Negl Trop Dis Journal subject: MEDICINA TROPICAL Year: 2020 Document type: Article Affiliation country: Japan Country of publication: United States

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Rabies / Rabies virus / Reagent Kits, Diagnostic Type of study: Diagnostic_studies / Evaluation_studies Limits: Animals Language: En Journal: PLoS Negl Trop Dis Journal subject: MEDICINA TROPICAL Year: 2020 Document type: Article Affiliation country: Japan Country of publication: United States