Monotropein alleviates H2O2induced inflammation, oxidative stress and apoptosis via NFκB/AP1 signaling.
Mol Med Rep
; 22(6): 4828-4836, 2020 Dec.
Article
in En
| MEDLINE
| ID: mdl-33173962
ABSTRACT
Aging is a major risk factor in cardiovascular disease (CVD). Oxidative stress and inflammation are involved in the pathogenesis of CVD, and are closely associated with senescent vascular endothelial cells. Monotropein (Mtp) exerts various bioactive roles, including antiinflammatory and antioxidative effects. The aim of the present study was to investigate the function of Mtp in senescent endothelial cells. An MTT assay was performed to evaluate the influence of Mtp on H2O2stimulated human umbilical vein endothelial cells (HUVECs). Senescent cells were assessed by determining the expression of senescenceassociated ßgalactosidase, high mobility group AThook 1 and DNA damage marker γH2A.X variant histone. Malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase (GSHPx) and proinflammatory cytokine concentrations were estimated using assay kits to evaluate the levels of oxidative stress and inflammation in HUVECs. The TUNEL assay was performed to identify apoptotic cells. Furthermore, the expression levels of endothelial cell adhesion factors, NFκB, activator protein1 (AP1) and apoptotic proteins were determined via western blotting. Mtp enhanced HUVEC viability following H2O2 stimulation. H2O2mediated increases in MDA, proinflammatory cytokine and endothelial cell adhesion factor levels were decreased by Mtp treatment, whereas Mtp reversed H2O2mediated downregulation of SOD and GSHPx activity. Furthermore, Mtp inhibited cell apoptosis, NFκB activation and AP1 expression in H2O2stimulated HUVECs; however, NFκB activator counteracted the antiinflammatory, antioxidative and antiapoptotic effects of Mtp. The present study indicated that Mtp ameliorated H2O2induced inflammation and oxidative stress potentially by regulating NFκB/AP1.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Apoptosis
/
Oxidative Stress
/
Iridoids
Type of study:
Prognostic_studies
/
Risk_factors_studies
Limits:
Humans
Language:
En
Journal:
Mol Med Rep
Year:
2020
Document type:
Article