Studying TGF-ß Signaling and TGF-ß-induced Epithelial-to-mesenchymal Transition in Breast Cancer and Normal Cells.

Transforming growth factor-ß (TGF-ß) is a secreted multifunctional factor that plays a key role in intercellular communication. Perturbations of TGF-ß signaling can lead to breast cancer. TGF-ß elicits its effects on proliferation and differentiation via specific cell surface TGF-ß type I and type II receptors (i.e., TßRI and TßRII) that contain an intrinsic serine/threonine kinase domain. Upon TGF-ß-induced heteromeric complex formation, activated TßRI elicits intracellular signaling by phosphorylating SMAD2 and SMAD3. These activated SMADs form heteromeric complexes with SMAD4 to regulate specific target genes, including plasminogen activation inhibitor 1 (PAI-1, encoded by the SERPINE1 gene). The induction of epithelial-to-mesenchymal transition (EMT) allows epithelial cancer cells at the primary site or during colonization at distant sites to gain an invasive phenotype and drive tumor progression. TGF-ß acts as a potent inducer of breast cancer invasion by driving EMT. Here, we describe systematic methods to investigate TGF-ß signaling and EMT responses using premalignant human MCF10A-RAS (M2) cells and mouse NMuMG epithelial cells as examples. We describe methods to determine TGF-ß-induced SMAD2 phosphorylation by Western blotting, SMAD3/SMAD4-dependent transcriptional activity using luciferase reporter activity and SERPINE1 target gene expression by quantitative real-time-polymerase chain reaction (qRT-PCR). In addition, methods are described to examine TGF-ß-induced EMT by measuring changes in morphology, epithelial and mesenchymal marker expression, filamentous actin staining and immunofluorescence staining of E-cadherin. Two selective small molecule TGF-ß receptor kinase inhibitors, GW788388 and SB431542, were used to block TGF-ß-induced SMAD2 phosphorylation, target genes and changes in EMT marker expression. Moreover, we describe the transdifferentiation of mesenchymal breast Py2T murine epithelial tumor cells into adipocytes. Methods to examine TGF-ß-induced signaling and EMT in breast cancer may contribute to new therapeutic approaches for breast cancer.