Your browser doesn't support javascript.
loading
CLA4 regulates leaf angle through multiple hormone signaling pathways in maize.
Dou, Dandan; Han, Shengbo; Cao, Liru; Ku, Lixia; Liu, Huafeng; Su, Huihui; Ren, Zhenzhen; Zhang, Dongling; Zeng, Haixia; Dong, Yahui; Liu, Zhixie; Zhu, Fangfang; Zhao, Qiannan; Xie, Jiarong; Liu, Yajing; Cheng, Haiyang; Chen, Yanhui.
Affiliation
  • Dou D; College of Agronomy, Synergetic Innovation Center of Henan Grain Crops and National Key Laboratory of Wheat and Maize Crop Science, Henan Agricultural University, Zhengdong New Area, Zhengzhou, Henan, China.
  • Han S; College of Agronomy, Synergetic Innovation Center of Henan Grain Crops and National Key Laboratory of Wheat and Maize Crop Science, Henan Agricultural University, Zhengdong New Area, Zhengzhou, Henan, China.
  • Cao L; Henan Academy of Agricultural Science, Zhengzhou, Henan, China.
  • Ku L; College of Agronomy, Synergetic Innovation Center of Henan Grain Crops and National Key Laboratory of Wheat and Maize Crop Science, Henan Agricultural University, Zhengdong New Area, Zhengzhou, Henan, China.
  • Liu H; College of Agronomy, Synergetic Innovation Center of Henan Grain Crops and National Key Laboratory of Wheat and Maize Crop Science, Henan Agricultural University, Zhengdong New Area, Zhengzhou, Henan, China.
  • Su H; College of Agronomy, Synergetic Innovation Center of Henan Grain Crops and National Key Laboratory of Wheat and Maize Crop Science, Henan Agricultural University, Zhengdong New Area, Zhengzhou, Henan, China.
  • Ren Z; College of Agronomy, Synergetic Innovation Center of Henan Grain Crops and National Key Laboratory of Wheat and Maize Crop Science, Henan Agricultural University, Zhengdong New Area, Zhengzhou, Henan, China.
  • Zhang D; College of Agronomy, Synergetic Innovation Center of Henan Grain Crops and National Key Laboratory of Wheat and Maize Crop Science, Henan Agricultural University, Zhengdong New Area, Zhengzhou, Henan, China.
  • Zeng H; College of Agronomy, Synergetic Innovation Center of Henan Grain Crops and National Key Laboratory of Wheat and Maize Crop Science, Henan Agricultural University, Zhengdong New Area, Zhengzhou, Henan, China.
  • Dong Y; College of Agronomy, Synergetic Innovation Center of Henan Grain Crops and National Key Laboratory of Wheat and Maize Crop Science, Henan Agricultural University, Zhengdong New Area, Zhengzhou, Henan, China.
  • Liu Z; College of Agronomy, Synergetic Innovation Center of Henan Grain Crops and National Key Laboratory of Wheat and Maize Crop Science, Henan Agricultural University, Zhengdong New Area, Zhengzhou, Henan, China.
  • Zhu F; College of Agronomy, Synergetic Innovation Center of Henan Grain Crops and National Key Laboratory of Wheat and Maize Crop Science, Henan Agricultural University, Zhengdong New Area, Zhengzhou, Henan, China.
  • Zhao Q; College of Agronomy, Synergetic Innovation Center of Henan Grain Crops and National Key Laboratory of Wheat and Maize Crop Science, Henan Agricultural University, Zhengdong New Area, Zhengzhou, Henan, China.
  • Xie J; College of Agronomy, Synergetic Innovation Center of Henan Grain Crops and National Key Laboratory of Wheat and Maize Crop Science, Henan Agricultural University, Zhengdong New Area, Zhengzhou, Henan, China.
  • Liu Y; College of Agronomy, Synergetic Innovation Center of Henan Grain Crops and National Key Laboratory of Wheat and Maize Crop Science, Henan Agricultural University, Zhengdong New Area, Zhengzhou, Henan, China.
  • Cheng H; College of Agronomy, Synergetic Innovation Center of Henan Grain Crops and National Key Laboratory of Wheat and Maize Crop Science, Henan Agricultural University, Zhengdong New Area, Zhengzhou, Henan, China.
  • Chen Y; College of Agronomy, Synergetic Innovation Center of Henan Grain Crops and National Key Laboratory of Wheat and Maize Crop Science, Henan Agricultural University, Zhengdong New Area, Zhengzhou, Henan, China.
J Exp Bot ; 72(5): 1782-1794, 2021 02 27.
Article in En | MEDLINE | ID: mdl-33270106
ABSTRACT
Leaf angle is an important agronomic trait in cereals and shares a close relationship with crop architecture and grain yield. Although it has been previously reported that ZmCLA4 can influence leaf angle, the underlying mechanism remains unclear. In this study, we used the Gal4-LexA/UAS system and transactivation analysis to demonstrate in maize (Zea mays) that ZmCLA4 is a transcriptional repressor that regulates leaf angle. DNA affinity purification sequencing (DAP-Seq) analysis revealed that ZmCLA4 mainly binds to promoters containing the EAR motif (CACCGGAC) as well as to two other motifs (CCGARGS and CDTCNTC) to inhibit the expression of its target genes. Further analysis of ZmCLA4 target genes indicated that ZmCLA4 functions as a hub of multiple plant hormone signaling pathways ZmCLA4 was found to directly bind to the promoters of multiple genes including ZmARF22 and ZmIAA26 in the auxin transport pathway, ZmBZR3 in the brassinosteroid signaling pathway, two ZmWRKY genes involved in abscisic acid metabolism, ZmCYP genes (ZmCYP75B1, ZmCYP93D1) related to jasmonic acid metabolism, and ZmABI3 involved in the ethylene response pathway. Overall, our work provides deep insights into the ZmCLA4 regulatory network in controlling leaf angle in maize.
Subject(s)
Key words
Fulltext
Add to My VHL
Print
XML
PubMed Links
Search on Google

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Plant Leaves / Zea mays Language: En Journal: J Exp Bot Journal subject: BOTANICA Year: 2021 Document type: Article Affiliation country: China
Fulltext
Add to My VHL
Print
XML
PubMed Links
Search on Google

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Plant Leaves / Zea mays Language: En Journal: J Exp Bot Journal subject: BOTANICA Year: 2021 Document type: Article Affiliation country: China