SPOCK1/SIX1axis promotes breast cancer progression by activating AKT/mTOR signaling.
Aging (Albany NY)
; 13(1): 1032-1050, 2020 12 03.
Article
in En
| MEDLINE
| ID: mdl-33293473
SPOCK1 is highly expressed in many types of cancer and has been recognized as a promoter of cancer progression. Its regulatory mechanism in breast cancer (BC) remains unclear. This study aimed to explore the precise function of SPOCK1 in BC progression and to identify the mechanism by which SPOCK1 is involved in cell proliferation and epithelial-mesenchymal transition (EMT). Immunohistochemistry (IHC) experiments and database analysis showed that high expression of SPOCK1 was positively associated with histological grade, lymph node metastasis (LN) and poor clinical prognosis in BC. A series of in vitro and in vivo assays elucidated that altering the SPOCK1 level led to distinct changes in BC cell proliferation and metastasis. Investigations of potential mechanisms revealed that SPOCK1 interacted with SIX1 to enhance cell proliferation, cell cycle progression and EMT by activating the AKT/mTOR pathway, whereas inhibition of the AKT/mTOR pathway or depletion of SIX1 reversed the effects of SPOCK1 overexpression. Furthermore, SPOCK1 and SIX1 were highly expressed in BC and might indicate poor prognoses. Altogether, the SPOCK1/SIX1 axis promoted BC progression by activating the AKT/mTOR pathway to accelerate cell proliferation and promote metastasis in BC, so the SPOCK1/SIX1 axis might be a promising clinical therapeutic target for preventing BC progression.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Proteoglycans
/
Breast Neoplasms
/
Adenocarcinoma
/
Homeodomain Proteins
/
Cell Proliferation
/
Epithelial-Mesenchymal Transition
Type of study:
Prognostic_studies
Limits:
Animals
/
Female
/
Humans
/
Middle aged
Language:
En
Journal:
Aging (Albany NY)
Journal subject:
GERIATRIA
Year:
2020
Document type:
Article
Affiliation country:
China
Country of publication:
United States