Alcohol dependence activates ventral tegmental area projections to central amygdala in male mice and rats.
Addict Biol
; 26(4): e12990, 2021 07.
Article
in En
| MEDLINE
| ID: mdl-33331103
ABSTRACT
The neural adaptations that occur during the transition to alcohol dependence are not entirely understood but may include a gradual recruitment of brain stress circuitry by mesolimbic reward circuitry that is activated during early stages of alcohol use. Here, we focused on dopaminergic and nondopaminergic projections from the ventral tegmental area (VTA), important for mediating acute alcohol reinforcement, to the central nucleus of the amygdala (CeA), important for alcohol dependence-related negative affect and escalated alcohol drinking. The VTA projects directly to the CeA, but the functional relevance of this circuit is not fully established. Therefore, we combined retrograde and anterograde tracing, anatomical, and electrophysiological experiments in mice and rats to demonstrate that the CeA receives input from both dopaminergic and nondopaminergic projection neurons primarily from the lateral VTA. We then used slice electrophysiology and fos immunohistochemistry to test the effects of alcohol dependence on activity and activation profiles of CeA-projecting neurons in the VTA. Our data indicate that alcohol dependence activates midbrain projections to the central amygdala, suggesting that VTA projections may trigger plasticity in the CeA during the transition to alcohol dependence and that this circuit may be involved in mediating behavioral dysregulation associated with alcohol dependence.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Ventral Tegmental Area
/
Alcoholism
/
Central Amygdaloid Nucleus
Limits:
Animals
Language:
En
Journal:
Addict Biol
Journal subject:
TRANSTORNOS RELACIONADOS COM SUBSTANCIAS
Year:
2021
Document type:
Article
Affiliation country:
United States