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siRNA biogenesis and advances in topically applied dsRNA for controlling virus infections in tomato plants.
Rego-Machado, Camila M; Nakasu, Erich Y T; Silva, João M F; Lucinda, Natália; Nagata, Tatsuya; Inoue-Nagata, Alice K.
Affiliation
  • Rego-Machado CM; Department of Plant Pathology, University of Brasília, Federal District, Brazil.
  • Nakasu EYT; Laboratory of Virology and Molecular Biology, Embrapa Vegetables, Federal District, Brazil.
  • Silva JMF; Laboratory of Virology and Molecular Biology, Embrapa Vegetables, Federal District, Brazil. erich.nakasu@embrapa.br.
  • Lucinda N; Department of Molecular Biology, University of Brasília, Federal District, Brazil.
  • Nagata T; Laboratory of Virology and Molecular Biology, Embrapa Vegetables, Federal District, Brazil.
  • Inoue-Nagata AK; Department of Plant Pathology, University of Florida, Florida, USA.
Sci Rep ; 10(1): 22277, 2020 12 17.
Article in En | MEDLINE | ID: mdl-33335295
A non-transgenic approach based on RNA interference was employed to induce protection against tomato mosaic virus (ToMV) infection in tomato plants. dsRNA molecules targeting the cp gene of ToMV were topically applied on plants prior to virus inoculation. Protection was dose-dependent and sequence-specific. While no protection was achieved when 0-16 µg dsRNA were used, maximum rates of resistance (60 and 63%) were observed in doses of 200 and 400 µg/plant, respectively. Similar rates were also obtained against potato virus Y when targeting its cp gene. The protection was quickly activated upon dsRNA application and lasted for up to 4 days. In contrast, no detectable antiviral response was triggered by the dsRNA from a begomovirus genome, suggesting the method is not effective against phloem-limited DNA viruses. Deep sequencing was performed to analyze the biogenesis of siRNA populations. Although long-dsRNA remained in the treated leaves for at least 10 days, its systemic movement was not observed. Conversely, dsRNA-derived siRNA populations (mainly 21- and 22-nt) were detected in non-treated leaves, which indicates endogenous processing and transport through the plant. Altogether, this study provides critical information for the development of novel tools against plant viruses; strengths and limitations inherent to the systems are discussed.
Subject(s)

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Plant Diseases / Virus Diseases / Solanum lycopersicum / Mosaic Viruses Language: En Journal: Sci Rep Year: 2020 Document type: Article Affiliation country: Brazil Country of publication: United kingdom

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Plant Diseases / Virus Diseases / Solanum lycopersicum / Mosaic Viruses Language: En Journal: Sci Rep Year: 2020 Document type: Article Affiliation country: Brazil Country of publication: United kingdom