CRISPR-Cas9 Ribonucleoprotein-Mediated Genomic Editing in Primary Innate Immune Cells.
STAR Protoc
; 1(3): 100113, 2020 12 18.
Article
in En
| MEDLINE
| ID: mdl-33377009
ABSTRACT
CRISPR-Cas9 genome engineering can be used to functionally investigate the complex mechanisms of immune system regulation. Decades of work have aimed to genetically reprogram innate immunity, but current approaches are inefficient or nonspecific, limiting their use. Here, we detail an optimized strategy for non-viral CRISPR-Cas9 ribonucleoprotein (cRNP) genomic editing of primary innate lymphocytes (ILCs) and myeloid lineage cells, resulting in high-efficiency editing of target gene expression from a single electroporation. For complete details on the use and execution of this protocol, please refer to Riggan et al. (2020).
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Electroporation
/
Gene Editing
/
Immunity, Innate
Limits:
Animals
/
Humans
Language:
En
Journal:
STAR Protoc
Year:
2020
Document type:
Article
Affiliation country:
United States