Accelerative effect of topical Zataria multiflora essential oil against infected wound model by modulating inflammation, angiogenesis, and collagen biosynthesis.
Pharm Biol
; 59(1): 1-10, 2021 Dec.
Article
in En
| MEDLINE
| ID: mdl-33378625
ABSTRACT
CONTEXT Zataria multiflora Boiss (Lamiaceae) essential oil (ZME) is believed to be a bactericide herbal medicine and might alleviate negative effects of infection. OBJECTIVE:
This study evaluates the effects of an ointment prepared from ZME (ZMEO) on infected wounds. MATERIALS ANDMETHODS:
A full-thickness excisional skin wound was surgically created in each mouse and inoculated with 5 × 107 suspension containing Pseudomonas aeruginosa and Staphylococcus aureus. The BALB/c mice (n = 72) were divided into four groups (1) negative control that received base ointment (NCG), (2) positive control that daily received Mupirocin® (MG), (3) therapeutic ointment containing 2% ZMEO and (4) therapeutic ointment containing 4% ZMEO, for 21 days. Wound contraction, total bacterial count, histopathological parameters, antioxidant activity, qRT-PCR analysis for expression of IL-1ß, TNF-α, VEGF, IGF-1, TGF-ß, IL-10, and FGF-2 mRNA levels were assessed on days 3, 7, and 14 following the wounding.RESULTS:
Topical administration of ZMEO significantly decreased the total bacterial count and wound area and also expression of IL-1ß and TNF-α compared to the control groups (p < 0.05) in all days. This could also increase significantly the expression of TGF-ß, IL-10 IGF-1, FGF-2, and VEGF, and also angiogenesis, fibroblasts, fibrocytes, epithelialization ratio, and collagen deposition and improve antioxidant status compared to the control group (p < 0.05). DISCUSSION ANDCONCLUSION:
ZMEO accelerated the healing process of infected wounds by shortening the inflammatory factors and increasing proliferative phase. Applying ZMEO only and/or in combination with chemical agents for the treatment of wound healing could be suggested.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Surgical Wound Infection
/
Wound Healing
/
Oils, Volatile
/
Collagen
/
Lamiaceae
/
Neovascularization, Pathologic
Type of study:
Prognostic_studies
Limits:
Animals
Language:
En
Journal:
Pharm Biol
Year:
2021
Document type:
Article
Affiliation country:
Iran