Dahlia variabilis cultivar 'Seattle' as a model plant for anthochlor biosynthesis.
Plant Physiol Biochem
; 159: 193-201, 2021 Feb.
Article
in En
| MEDLINE
| ID: mdl-33385702
ABSTRACT
We investigated the bi-colored dahlia cultivar 'Seattle', which exhibits bright yellow petals with white tips, for its potential use as a model system for studies of the anthochlor biosynthesis. The yellow base contained high amounts of the 6'-deoxychalcones and the structurally related 4-deoxyaurones, as well as flavones. In contrast, only traces of anthochlors and flavones were detected in the white tips. No anthocyanins, flavonols, flavanones or dihydroflavonols were found in the petals. Gene expression studies indicated that the absence of anthocyanins in the petals is caused by a lack of flavanone 3-hydroxylase (FHT) expression, which is accompanied by a lack of expression of the bHLH transcription factor IVS. Expression of other genes involved in anthocyanidin biosynthesis such as dihydroflavonol 4-reductase (DFR) and anthocyanidin synthase (ANS) was not affected. The yellow and white petal parts showed significant differences in the expression of chalcone synthase 2 (CHS2), which is sufficient to explain the absence of yellow pigments in the white tips. Transcriptomes of both petal parts were de novo assembled and three candidate genes for chalcone reductase (CHR) were identified. None of them showed a significantly higher expression in the yellow base compared to the white tips. In summary, it was shown that the bicolouration is most likely caused by a bottleneck in chalcone formation in the white tip. The relative prevalence of flavones compared to the anthochlors in the white tips could be an indication for the presence of a so far unknown differentially expressed CHR.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Pigments, Biological
/
Gene Expression Regulation, Plant
/
Dahlia
/
Models, Biological
Type of study:
Prognostic_studies
/
Risk_factors_studies
Language:
En
Journal:
Plant Physiol Biochem
Journal subject:
BIOQUIMICA
/
BOTANICA
Year:
2021
Document type:
Article
Affiliation country:
Austria