In Vivo Studies of Drug BBB Transport: Translational Challenges and the Role of Brain Imaging.
Handb Exp Pharmacol
; 273: 223-244, 2022.
Article
in En
| MEDLINE
| ID: mdl-33387067
ABSTRACT
Drug delivery to the brain is challenging to study due to the complexity of the barriers of the central nervous system (CNS). The present chapter describes and compares experimental methods such as microdialysis, two-photon laser scanning fluorescence microscopy and positron emission tomography (PET) that can be used for in vivo studies of drug transport across the blood-brain barrier (BBB). The selection of appropriate method is based on the research question, and the different methods will in most cases provide complementary information. Attention is also given to the fact that the BBB might undergo changes in integrity, protein expression and other morphological alterations as a result of disease. The use of animal models of human disease is therefore also discussed. Special emphasis is given to translational aspects of the different methods and readouts.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Brain
/
Blood-Brain Barrier
Limits:
Animals
/
Humans
Language:
En
Journal:
Handb Exp Pharmacol
Year:
2022
Document type:
Article
Affiliation country:
Sweden