Upper Respiratory Microbiota in Relation to Ear and Nose Health Among Australian Aboriginal and Torres Strait Islander Children.
J Pediatric Infect Dis Soc
; 10(4): 468-476, 2021 Apr 30.
Article
in En
| MEDLINE
| ID: mdl-33393596
ABSTRACT
BACKGROUND:
We explored the nasal microbiota in Indigenous Australian children in relation to ear and nasal health.METHODS:
In total, 103 Indigenous Australian children aged 2-7 years (mean 4.7 years) were recruited from 2 Queensland communities. Children's ears, nose, and throats were examined and upper respiratory tract (URT) swabs collected. Clinical histories were obtained from parents/medical records. URT microbiota were characterized using culturomics with Matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) identification. Real-time PCR was used to quantify otopathogen (Haemophilus influenzae, Streptococcus pneumoniae, and Moraxella catarrhalis) loads and detect respiratory viruses. Data were analyzed using beta diversity measures, regression modeling, and a correlation network analysis.RESULTS:
Children with historical/current otitis media (OM) or URT infection (URTI) had higher nasal otopathogen detection and loads and rhinovirus detection compared with healthy children (all P < .04). Children with purulent rhinorrhea had higher nasal otopathogen detection and loads and rhinovirus detection (P < .04) compared with healthy children. High otopathogen loads were correlated in children with historical/current OM or URTI, whereas Corynebacterium pseudodiphtheriticum and Dolosigranulum pigrum were correlated in healthy children.CONCLUSIONS:
Corynebacterium pseudodiphtheriticum and D. pigrum are associated with URT and ear health. The importance of the main otopathogens in URT disease/OM was confirmed, and their role relates to co-colonization and high otopathogens loads.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Otitis Media
/
Carnobacteriaceae
/
Microbiota
Type of study:
Prognostic_studies
Limits:
Child
/
Humans
Country/Region as subject:
Oceania
Language:
En
Journal:
J Pediatric Infect Dis Soc
Year:
2021
Document type:
Article
Affiliation country:
Australia