PolyIC Induces Retinoic Acid-inducible Gene-I and Melanoma Differentiation-associated Gene 5 and Modulates Inflammation in Podocytes.
In Vivo
; 35(1): 147-153, 2021.
Article
in En
| MEDLINE
| ID: mdl-33402460
ABSTRACT
BACKGROUND/AIM:
Viral infection often exacerbates proteinuria, which has been suggested to be due to antiviral responses of podocytes. We examined the effect of polyinosinic-polycytidylic acid (polyIC) on the expression of retinoic acid-inducible gene-I (RIG-I) and melanoma differentiation-associated gene 5 (MDA5) in differentiated human podocytes in culture. MATERIALS ANDMETHODS:
The podocytes were treated with 2 ng/ml to 500 µg/ml of polyIC for 3 to 36 h, and also transfected with siRNA against RIG-I and MDA5. F-actin staining was performed to assess actin reorganization.RESULTS:
PolyIC induced the expression of RIG-I and MDA5 in dose- and time-dependent manner, accompanied with interferon-ß (IFN-ß) and interleukin-6 (IL-6) up-regulation and actin reorganization. Temporal knockdown of RIG-I by siRNA decreased IFN-ß expression, while MDA5 siRNA inhibited IFN-ß and IL-6 expression. Actin reorganization was attenuated by RIG-I and MDA5 knockdown.CONCLUSION:
RIG-I and MDA5 may play a role in the antiviral responses of podocytes.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Podocytes
/
Melanoma
Type of study:
Risk_factors_studies
Limits:
Humans
Language:
En
Journal:
In Vivo
Journal subject:
NEOPLASIAS
Year:
2021
Document type:
Article
Affiliation country:
Japan
Publication country:
GR
/
GRECIA
/
GREECE
/
GRÉCIA