Luminescent Amphiphilic Aminoglycoside Probes to Study Transfection.
Chembiochem
; 22(9): 1563-1567, 2021 05 04.
Article
in En
| MEDLINE
| ID: mdl-33410196
We report the characterization of amphiphilic aminoglycoside conjugates containing luminophores with aggregation-induced emission properties as transfection reagents. These inherently luminescent transfection vectors are capable of binding plasmid DNA through electrostatic interactions; this binding results in an emission "on" signal due to restriction of intramolecular motion of the luminophore core. The luminescent cationic amphiphiles effectively transferred plasmid DNA into mammalian cells (HeLa, HEK 293T), as proven by expression of a red fluorescent protein marker. The morphologies of the aggregates were investigated by microscopy as well as ζ-potential and dynamic light-scattering measurements. The transfection efficiencies using luminescent cationic amphiphiles were similar to that of the gold-standard transfection reagent Lipofectamine® 2000.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Transfection
/
Aminoglycosides
Limits:
Animals
/
Humans
Language:
En
Journal:
Chembiochem
Journal subject:
BIOQUIMICA
Year:
2021
Document type:
Article
Affiliation country:
Germany
Country of publication:
Germany