miRNA199b3p suppresses growth and progression of ovarian cancer via the CHK1/Ecadherin/EMT signaling pathway by targeting ZEB1.
Oncol Rep
; 45(2): 569-581, 2021 02.
Article
in En
| MEDLINE
| ID: mdl-33416170
Ovarian cancer is one of the most common gynecological malignancies and its pathogenesis and progression are regulated by multiple genes. MicroRNAs (miRNAs) are endogenous noncoding RNAs that regulate body function by altering posttranscriptional gene expression. Previous studies have suggested that miRNAs are closely associated with the pathogenesis and progression of several malignancies, including breast cancer, hepatocellular carcinoma and glioma, among others. Therefore, miRNAs are promising novel targets for the diagnosis, treatment and determination of prognostic factors in patients with ovarian cancer. In the present study, the role of miRNA133b3p in ovarian cancer progression and its possible mechanism of action were investigated. The results demonstrated that the expression of miRNA199b3p and zinc finger Ebox binding homeobox (ZEB)1 were increased in patients with ovarian cancer. The overall survival (OS) and diseasefree survival (DFS) of patients with ovarian cancer and high miRNA199b3p expression were prolonged compared with those of patients with low miRNA199b3p expression. Additionally, the OS and DFS of patients with ovarian cancer and low ZEB1 expression were longer compared with those of patients with high ZEB1 expression. Furthermore, miRNA199b3p overexpression reduced cell proliferation and promoted apoptosis in an in vitro model of ovarian cancer. miRNA199b3p overexpression also suppressed ZEB1 and checkpoint kinase 1 expression and induced Ecadherin expression and epithelialtomesenchymal transition in this model. Furthermore, the effects of miRNA199b3pmediated apoptosis and migration were attenuated by ZEB1 and Ecadherin, respectively. The results of the present study indicated that miRNA199b3p suppressed ovarian cancer progression by targeting ZEB1, which may represent a promising therapeutic target for ovarian cancer.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Ovarian Neoplasms
/
MicroRNAs
/
Zinc Finger E-box-Binding Homeobox 1
/
Neoplasm Recurrence, Local
Type of study:
Observational_studies
/
Prognostic_studies
/
Risk_factors_studies
Limits:
Animals
/
Female
/
Humans
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Male
/
Middle aged
Language:
En
Journal:
Oncol Rep
Journal subject:
NEOPLASIAS
Year:
2021
Document type:
Article
Country of publication:
Greece