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Reorganized Genomic Taxonomy of Francisellaceae Enables Design of Robust Environmental PCR Assays for Detection of Francisella tularensis.
Öhrman, Caroline; Sahl, Jason W; Sjödin, Andreas; Uneklint, Ingrid; Ballard, Rebecca; Karlsson, Linda; McDonough, Ryelan F; Sundell, David; Soria, Kathleen; Bäckman, Stina; Chase, Kitty; Brindefalk, Björn; Sozhamannan, Shanmuga; Vallesi, Adriana; Hägglund, Emil; Ramirez-Paredes, Jose Gustavo; Thelaus, Johanna; Colquhoun, Duncan; Myrtennäs, Kerstin; Birdsell, Dawn; Johansson, Anders; Wagner, David M; Forsman, Mats.
Affiliation
  • Öhrman C; CBRN Defence and Security, Swedish Defence Research Agency, FOI, SE 901 82 Umeå, Sweden.
  • Sahl JW; Pathogen and Microbiome Institute, Northern Arizona University, Flagstaff, AZ 86011, USA.
  • Sjödin A; CBRN Defence and Security, Swedish Defence Research Agency, FOI, SE 901 82 Umeå, Sweden.
  • Uneklint I; CBRN Defence and Security, Swedish Defence Research Agency, FOI, SE 901 82 Umeå, Sweden.
  • Ballard R; Pathogen and Microbiome Institute, Northern Arizona University, Flagstaff, AZ 86011, USA.
  • Karlsson L; CBRN Defence and Security, Swedish Defence Research Agency, FOI, SE 901 82 Umeå, Sweden.
  • McDonough RF; Pathogen and Microbiome Institute, Northern Arizona University, Flagstaff, AZ 86011, USA.
  • Sundell D; CBRN Defence and Security, Swedish Defence Research Agency, FOI, SE 901 82 Umeå, Sweden.
  • Soria K; Pathogen and Microbiome Institute, Northern Arizona University, Flagstaff, AZ 86011, USA.
  • Bäckman S; CBRN Defence and Security, Swedish Defence Research Agency, FOI, SE 901 82 Umeå, Sweden.
  • Chase K; US Army Medical Research Institute, Fort Detrick, MD 21702, USA.
  • Brindefalk B; CBRN Defence and Security, Swedish Defence Research Agency, FOI, SE 901 82 Umeå, Sweden.
  • Sozhamannan S; Logistics Management Institute supporting Defense Biological Product Assurance Office (DBPAO) Joint Project Lead, CBRND Enabling Biotechnologies (JPL CBRND EB), Frederick, MD 21702, USA.
  • Vallesi A; School of Biosciences and Veterinary Medicine, University of Camerino, 62032 Camerino, Italy.
  • Hägglund E; CBRN Defence and Security, Swedish Defence Research Agency, FOI, SE 901 82 Umeå, Sweden.
  • Ramirez-Paredes JG; Ridgeway Biologicals Limited a Ceva Santé Animale Company, Units 1-3 Old Station Business Park, Compton, Berkshire, England RG20 6NE, UK.
  • Thelaus J; CBRN Defence and Security, Swedish Defence Research Agency, FOI, SE 901 82 Umeå, Sweden.
  • Colquhoun D; Fish Health Research Group, Norwegian Veterinary Institute, Oslo, Pb 750 Sentrum, 23 N-0106 Oslo, Norway.
  • Myrtennäs K; CBRN Defence and Security, Swedish Defence Research Agency, FOI, SE 901 82 Umeå, Sweden.
  • Birdsell D; Pathogen and Microbiome Institute, Northern Arizona University, Flagstaff, AZ 86011, USA.
  • Johansson A; Department of Clinical Microbiology, Clinical Bacteriology, and Laboratory for Molecular Infection Medicine Sweden (MIMS), Umeå University, SE-901 85 Umeå, Sweden.
  • Wagner DM; Pathogen and Microbiome Institute, Northern Arizona University, Flagstaff, AZ 86011, USA.
  • Forsman M; CBRN Defence and Security, Swedish Defence Research Agency, FOI, SE 901 82 Umeå, Sweden.
Microorganisms ; 9(1)2021 Jan 11.
Article in En | MEDLINE | ID: mdl-33440900
ABSTRACT
In recent years, an increasing diversity of species has been recognized within the family Francisellaceae. Unfortunately, novel isolates are sometimes misnamed in initial publications or multiple sources propose different nomenclature for genetically highly similar isolates. Thus, unstructured and occasionally incorrect information can lead to confusion in the scientific community. Historically, detection of Francisella tularensis in environmental samples has been challenging due to the considerable and unknown genetic diversity within the family, which can result in false positive results. We have assembled a comprehensive collection of genome sequences representing most known Francisellaceae species/strains and restructured them according to a taxonomy that is based on phylogenetic structure. From this structured dataset, we identified a small number of genomic regions unique to F. tularensis that are putatively suitable for specific detection of this pathogen in environmental samples. We designed and validated specific PCR assays based on these genetic regions that can be used for the detection of F. tularensis in environmental samples, such as water and air filters.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Type of study: Diagnostic_studies / Prognostic_studies Language: En Journal: Microorganisms Year: 2021 Document type: Article Affiliation country: Sweden
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Full text: 1 Collection: 01-internacional Database: MEDLINE Type of study: Diagnostic_studies / Prognostic_studies Language: En Journal: Microorganisms Year: 2021 Document type: Article Affiliation country: Sweden