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Apocryphal FADS2 activity promotes fatty acid diversification in cancer.
Young, Reuben S E; Bowman, Andrew P; Williams, Elizabeth D; Tousignant, Kaylyn D; Bidgood, Charles L; Narreddula, Venkateswara R; Gupta, Rajesh; Marshall, David L; Poad, Berwyck L J; Nelson, Colleen C; Ellis, Shane R; Heeren, Ron M A; Sadowski, Martin C; Blanksby, Stephen J.
Affiliation
  • Young RSE; School of Chemistry and Physics, Queensland University of Technology, Brisbane, QLD 4000, Australia.
  • Bowman AP; M4I, The Maastricht MultiModal Molecular Imaging Institute, Division of Imaging Mass Spectrometry, Maastricht University, Universiteitssingel 50, 6229 ER Maastricht, the Netherlands.
  • Williams ED; Australian Prostate Cancer Research Centre-Queensland, Institute of Health and Biomedical Innovation, School of Biomedical Sciences, Faculty of Health, Queensland University of Technology (QUT), Princess Alexandra Hospital, Translational Research Institute, Brisbane, QLD 4000, Australia.
  • Tousignant KD; Australian Prostate Cancer Research Centre-Queensland, Institute of Health and Biomedical Innovation, School of Biomedical Sciences, Faculty of Health, Queensland University of Technology (QUT), Princess Alexandra Hospital, Translational Research Institute, Brisbane, QLD 4000, Australia.
  • Bidgood CL; Australian Prostate Cancer Research Centre-Queensland, Institute of Health and Biomedical Innovation, School of Biomedical Sciences, Faculty of Health, Queensland University of Technology (QUT), Princess Alexandra Hospital, Translational Research Institute, Brisbane, QLD 4000, Australia.
  • Narreddula VR; School of Chemistry and Physics, Queensland University of Technology, Brisbane, QLD 4000, Australia.
  • Gupta R; Central Analytical Research Facility, Institute for Future Environments, Queensland University of Technology, 2 George St., Brisbane, QLD 4000, Australia.
  • Marshall DL; Central Analytical Research Facility, Institute for Future Environments, Queensland University of Technology, 2 George St., Brisbane, QLD 4000, Australia.
  • Poad BLJ; School of Chemistry and Physics, Queensland University of Technology, Brisbane, QLD 4000, Australia; Central Analytical Research Facility, Institute for Future Environments, Queensland University of Technology, 2 George St., Brisbane, QLD 4000, Australia.
  • Nelson CC; Australian Prostate Cancer Research Centre-Queensland, Institute of Health and Biomedical Innovation, School of Biomedical Sciences, Faculty of Health, Queensland University of Technology (QUT), Princess Alexandra Hospital, Translational Research Institute, Brisbane, QLD 4000, Australia.
  • Ellis SR; M4I, The Maastricht MultiModal Molecular Imaging Institute, Division of Imaging Mass Spectrometry, Maastricht University, Universiteitssingel 50, 6229 ER Maastricht, the Netherlands; Molecular Horizons and School of Chemistry and Molecular Bioscience, University of Wollongong, Wollongong, NSW 2522,
  • Heeren RMA; M4I, The Maastricht MultiModal Molecular Imaging Institute, Division of Imaging Mass Spectrometry, Maastricht University, Universiteitssingel 50, 6229 ER Maastricht, the Netherlands.
  • Sadowski MC; Australian Prostate Cancer Research Centre-Queensland, Institute of Health and Biomedical Innovation, School of Biomedical Sciences, Faculty of Health, Queensland University of Technology (QUT), Princess Alexandra Hospital, Translational Research Institute, Brisbane, QLD 4000, Australia; Institute o
  • Blanksby SJ; School of Chemistry and Physics, Queensland University of Technology, Brisbane, QLD 4000, Australia; Central Analytical Research Facility, Institute for Future Environments, Queensland University of Technology, 2 George St., Brisbane, QLD 4000, Australia. Electronic address: stephen.blanksby@qut.edu
Cell Rep ; 34(6): 108738, 2021 02 09.
Article in En | MEDLINE | ID: mdl-33567271
ABSTRACT
Canonical fatty acid metabolism describes specific enzyme-substrate interactions that result in products with well-defined chain lengths, degree(s), and positions of unsaturation. Deep profiling of lipids across a range of prostate cancer cell lines reveals a variety of fatty acids with unusual site(s) of unsaturation that are not described by canonical pathways. The structure and abundance of these unusual lipids correlate with changes in desaturase expression and are strong indicators of cellular phenotype. Gene silencing and stable isotope tracing demonstrate that direct Δ6 and Δ8 desaturation of 140 (myristic), 160 (palmitic), and 180 (stearic) acids by FADS2 generate new families of unsaturated fatty acids (including n-8, n-10, and n-12) that have rarely-if ever-been reported in human-derived cells. Isomer-resolved lipidomics reveals the selective incorporation of these unusual fatty acids into complex structural lipids and identifies their presence in cancer tissues, indicating functional roles in membrane structure and signaling.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Prostatic Neoplasms / Signal Transduction / Fatty Acid Desaturases / Fatty Acids / Neoplasm Proteins Limits: Humans / Male Language: En Journal: Cell Rep Year: 2021 Document type: Article Affiliation country: Australia Publication country: EEUU / ESTADOS UNIDOS / ESTADOS UNIDOS DA AMERICA / EUA / UNITED STATES / UNITED STATES OF AMERICA / US / USA

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Prostatic Neoplasms / Signal Transduction / Fatty Acid Desaturases / Fatty Acids / Neoplasm Proteins Limits: Humans / Male Language: En Journal: Cell Rep Year: 2021 Document type: Article Affiliation country: Australia Publication country: EEUU / ESTADOS UNIDOS / ESTADOS UNIDOS DA AMERICA / EUA / UNITED STATES / UNITED STATES OF AMERICA / US / USA