Modulation of the inflammatory environment by spermatozoa through regulation of transforming growth factor beta in porcine uterine epithelial cells.
Reprod Biol
; 21(2): 100484, 2021 Jun.
Article
in En
| MEDLINE
| ID: mdl-33601292
ABSTRACT
This study investigated the changes in the mRNA expression of transforming growth factor beta (TGF-ß), plasminogen activators (PAs), and interleukin (IL) caused by sperm, as well as the regulatory mechanism of PA activity through TGF-ß, in porcine uterine epithelial cells. The cells were isolated from the uterine horn of pig and co-incubated with Percoll-separated boar sperm (45% or 90%), or TGF-ß for 24 h. The mRNA expression of TGF-ß isoforms (TGF-ß1, 2 and 3) and their receptors (TGF-ß R1 and R2), PAs (urokinase-type, uPA; tissue-type, tPA; uPA receptor, uPAR; type 1 PA inhibitor, PAI-1), IL-6 and IL-8 was analyzed using real-time PCR. Supernatant was used to measure PA activity. Co-incubation with sperm from the 90% Percoll layer increased TGF-ß1 mRNA, whereas TGF-ß2 and TGF-ß3 were decreased (P < 0.05). However, both TGF-ßRs were not changed by the presence of the spermatozoa. Expression of tPA, PAI-1, IL-6, and IL-8 mRNA was down-regulated by 90% Percoll-separated sperm (P < 0.05), and sperm from 45% Percoll increased uPA expression (P < 0.05). TGF-ß decreased tPA and IL-8 mRNA expression, and increased uPAR and PAI-1 mRNA (P < 0.05). The suppressive effect of TGF-ß on PA activity was blocked by Smad2/3 and JNK1/2 signaling inhibitors (P < 0.05). In conclusion, sperm separated in 90% in porcine uterus could suppressed inflammation via modulation of TGF-ß and down-regulation of PAs and ILs. Therefore, the regulatory mechanism of inflammation by sperm in the porcine uterus could be associated with interactions between numerous cytokines including TGF-ß.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Spermatozoa
/
Swine
/
Uterus
/
Transforming Growth Factor beta
/
Epithelial Cells
Limits:
Animals
Language:
En
Journal:
Reprod Biol
Journal subject:
MEDICINA REPRODUTIVA
Year:
2021
Document type:
Article