Identification of the internal ribosome entry sites in the 5'untranslated region of the cfos gene.
Int J Mol Med
; 47(4)2021 04.
Article
in En
| MEDLINE
| ID: mdl-33604677
ABSTRACT
The Fos protooncogene, activator protein1 (AP1) transcription factor subunit (cfos) gene, a member of the immediate early gene family, encodes cFos, which is a subunit of the AP1 transcription factor. The present study aimed to investigate the mechanism by which the translation efficiency of cfos mRNA is upregulated when cellular protein synthesis is shut off. The result of western blotting revealed that the protein expression levels of cFos were increased in rhabdomyosarcoma cells infected with enterovirus 71 (EV71) compared with uninfected cells. PCR was used to get the cfos 5'untranslated region (UTR). The luciferase assay of a bicistronic vector containing the cfos 5'UTR revealed that the cfos 5'UTR contains an internal ribosome entry site (IRES) sequence and a 175 nucleotide sequence (between 31 and 205 nt) that is essential for IRES activity. Analysis of potential IRES transacting factors revealed that poly(C)binding protein 2 (PCBP2) negatively regulated the activity of the cfos IRES, whereas the La autoantigen (La) positively regulated its activity. The results of RNAprotein immunoprecipitation demonstrated that both PCBP2 and La bound to the cfos 5'UTR. Furthermore, the IRES activity of in vitrotranscribed cfos mRNA was upregulated during EV71 infection. The present study suggested a mechanism for the effect of viral infection on host genes, and provided a novel target for gene translation regulation.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Gene Expression Regulation
/
Proto-Oncogene Proteins c-fos
/
Genes, fos
/
5' Untranslated Regions
/
Internal Ribosome Entry Sites
Type of study:
Diagnostic_studies
/
Prognostic_studies
Limits:
Humans
Language:
En
Journal:
Int J Mol Med
Journal subject:
BIOLOGIA MOLECULAR
/
GENETICA MEDICA
Year:
2021
Document type:
Article
Publication country:
GR
/
GRECIA
/
GREECE
/
GRÉCIA