Morphological analyses of nephrin expression in progressive glomerulonephropathy of common marmosets.
J Toxicol Pathol
; 34(1): 83-88, 2021 Jan.
Article
in En
| MEDLINE
| ID: mdl-33627947
ABSTRACT
In this study, we focused on nephrin, one of the key molecules within the slit diaphragm of podocytes, as although there have been reports on its expression in humans and rats, their presence in common marmosets has not been reported. We investigated nephrin expression and changes in glomeruli, depending on the development of spontaneous progressive glomerulonephropathy in common marmosets. Nineteen common marmosets at two to ten years of age were evaluated. The kidney was examined by microscopy with hematoxylin and eosin and immunohistochemical staining for nephrin. The lesions were classified into three grades according to a renal lesion grading system reported previously. The nephrin-positive area was measured by morphometric analysis, and the nephrin-positive ratio was calculated. Nephrin expression was observed along the glomerular capillary loop in a continuous linear pattern in renal lesion grades 0 to 2 and either discontinuous linear or coarse granular pattern in grade 3. Nephrin expression tended to decrease significantly depending on the grade of renal lesions. Alteration in nephrin expression has been suggested to play an important role in the progression of renal lesions.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Language:
En
Journal:
J Toxicol Pathol
Year:
2021
Document type:
Article
Affiliation country:
Japan