MiRNA-621 exerts tumor suppressor function in gastric adenocarcinoma by targeting AURKA/GSK-3ß pathway.
Acta Biochim Pol
; 68(1): 91-98, 2021 Feb 25.
Article
in En
| MEDLINE
| ID: mdl-33629578
ABSTRACT
Gastric adenocarcinoma is a major challenge to human health worldwide. Abnormal expression of miR-621 was found in many types of cancer. This research aimed to investigate the effects and detailed molecular mechanisms of miR-621 on gastric adenocarcinoma progression. The present study first showed that miR-621 was downregulated in gastric cancer patients, and its expression level was correlated with tumor size. MiR-621 overexpression inhibited viability, colony formation and proliferation of gastric cancer cells. AURKA was identified as a direct target of miR-621. AURKA knockdown induced decrease of p-GSK-3ß/GSK-3ß ratio and increase of p-ß-catenin/ß-catenin ratio which confirmed that AURKA positively regulated GSK-3ß phosphorylation. AURKA knockdown also inhibited proliferation of gastric adenocarcinoma cells. AURKA expression was negatively correlated with miR-621 level. In addition, AURKA overexpression reversed the effect of miR-621 on the growth of cancer cells. Taken together, our results suggest that miR-621 is an important tumor suppressor in gastric cancer and could be a promising target for the cancer treatment.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Stomach Neoplasms
/
Adenocarcinoma
/
Signal Transduction
/
Genes, Tumor Suppressor
/
MicroRNAs
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Aurora Kinase A
/
Glycogen Synthase Kinase 3 beta
Limits:
Female
/
Humans
/
Male
/
Middle aged
Language:
En
Journal:
Acta Biochim Pol
Year:
2021
Document type:
Article
Affiliation country:
China