Purification and characterization of DSIP-like material from ovine pineal glands: possible peptide-protein interaction.
J Pineal Res
; 5(2): 161-77, 1988.
Article
in En
| MEDLINE
| ID: mdl-3367267
ABSTRACT
The nonapeptide delta-sleep-inducing peptide (DSIP) has been isolated from venous blood of rabbits induced to sleep. Numerous reports have described sleep as well as extra-sleep effects. Radiochemical and immunochemical data suggest a relationship of DSIP with the pineal gland supported by interactions of this peptide with pineal functions such as the serotonin N-acetyltransferase activity. In order to demonstrate the natural occurrence of DSIP-like material associated with high Mr proteins in the ovine pineal, organs were water-extracted and fractionated by ultrafiltration and gel filtration. Radioimmunoassay (RIA) for DSIP-like fragments of the fractions revealed considerable amounts of pineal DSIP-like immunoreactivity (DSIP-LI) apparently existing in small as well as large molecular forms. Acidification of large DSIP-LI forms resulted in the elution from Sephadex G-50 of Mr less than or equal to 1,000 DSIP-like material. This free DSIP-LI form coeluted with the synthetic DSIP nonapeptide from microBondapak C18 on high-performance liquid chromatography. The results, therefore, appear to indicate the presence of a (biospecific) noncovalent intermolecular interaction of DSIP (1-9) with proteins (Mr greater than or equal to 10,000) of the ovine pineal gland.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Pineal Gland
/
Delta Sleep-Inducing Peptide
Limits:
Animals
Language:
En
Journal:
J Pineal Res
Journal subject:
ENDOCRINOLOGIA
Year:
1988
Document type:
Article
Affiliation country:
Netherlands