ACC deaminase-producing Ensifer adhaerens KS23 enhances proximate nutrient of Pisum sativum L. cultivated in high altitude.

ABSTRACT

A phytohormone producing, N2-fixing and 1-aminocyclopropane-1-carboxylate (ACC) deaminase synthesizing bacterium Ensifer adhaerens KS23 effectively increased the yield and nutritional contents of Pisum sativum var. Arkel. The isolate KS23 showed positive ACC deaminase activity with 174.2 (nmol of α-ketobutyrate/g-1 biomass½ h-1) a 9.7-fold increase in glutathione S-transferase activity. The proximate analysis exhibited an increased yield of protein (21.45%), carbohydrate (38.90%), sulphur (29.94%) starch (27.52%), total ash (35.57%), fat content (27.5%), nitrogen (24.06%) and hydrogen (17.91%) in treated seeds of P. sativum as compared to untreated crop seeds in field trials at Srikot village, Srinagar-246,174 (Garhwal) India. The most desirable essential and non-essential amino-acids content was also enhanced simultaneously by E. adhaerens KS23 as compared to non-treated crop seeds. This study revealed the enhancement of various nutritional contents resulting in quality improvement and an increase in growth productivity of pea. This study provides an encouraging result that may benefit the marginal income of farmers belonging mainly to hilly regions who are dependent on traditional methods of farming and thus improving their economy.