Selective dendritic localization of mRNA in Drosophila mushroom body output neurons.
Elife
; 102021 03 16.
Article
in En
| MEDLINE
| ID: mdl-33724180
ABSTRACT
Memory-relevant neuronal plasticity is believed to require local translation of new proteins at synapses. Understanding this process requires the visualization of the relevant mRNAs within these neuronal compartments. Here, we used single-molecule fluorescence in situ hybridization to localize mRNAs at subcellular resolution in the adult Drosophila brain. mRNAs for subunits of nicotinic acetylcholine receptors and kinases could be detected within the dendrites of co-labeled mushroom body output neurons (MBONs) and their relative abundance showed cell specificity. Moreover, aversive olfactory learning produced a transient increase in the level of CaMKII mRNA within the dendritic compartments of the γ5ß'2a MBONs. Localization of specific mRNAs in MBONs before and after learning represents a critical step towards deciphering the role of dendritic translation in the neuronal plasticity underlying behavioral change in Drosophila.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
RNA, Messenger
/
Mushroom Bodies
/
Dendrites
/
Drosophila
/
Neurons
Limits:
Animals
Language:
En
Journal:
Elife
Year:
2021
Document type:
Article
Affiliation country:
United kingdom