Functional Activity of Non-Proliferating Mesenchymal Stromal Cells Cultured at Different Densities.
Bull Exp Biol Med
; 170(4): 537-543, 2021 Feb.
Article
in En
| MEDLINE
| ID: mdl-33725246
ABSTRACT
We analyzed the state of intracellular compartments and production of cytokines in MSC depending on the culture density. MSC were growth-arrested with mitomycin C and seeded at a density of 300-7000 cell/cm2. MSC in low-density cultures had 2-fold higher levels of transmembrane mitochondrial potential (MitoTracker Red) and endogenous ROS (CMH2DCFDA), lysosomal compartments were less acidified (LysoTracker Green DND26), the production of immunoregulatory and angiogenic mediators VEGF, IL-6, IL-8, MCP-1, TGF-ß was more intensive. It was assumed that culture density can be an effective tool for phenotypic polarization of MSC providing directional changes in their properties.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Mesenchymal Stem Cells
Limits:
Humans
Language:
En
Journal:
Bull Exp Biol Med
Year:
2021
Document type:
Article
Affiliation country:
RUSSIA