miR5413p inhibits the viability and migration of vascular smooth muscle cells via targeting STIM1.
Mol Med Rep
; 23(5)2021 05.
Article
in En
| MEDLINE
| ID: mdl-33760103
The transformation of vascular smooth muscle cells (VSMCs) into the proliferative migratory phenotype in the plaque area contributes to stable plaque formation and facilitates the pathogenesis of atherosclerosis. Stromal interaction molecule 1 (STIM1) has been identified to promote the proliferation of VSMCs, suggesting that STIM1 may be a potent target for the prevention and treatment of atherosclerosis. Bioinformatics analysis has previously predicted STIM1 as a target of microRNA (miR)5413p. The present study aimed to determine the effect of the miR5413p/STIM1 axis on the progression of atherosclerosis in vitro. Oxidized lowdensity lipoprotein (oxLDL)treated VSMCs were used as an in vitro atherosclerosis model. Cell Counting Kit8 and Transwell migration assays were used to analyze cell viability and migration, respectively. Reverse transcriptionquantitative PCR and western blotting were applied to measure mRNA and protein expression levels, respectively. The association between miR5413p and STIM1 was detected using a dual luciferase gene reporter assay. The results of the present study revealed that oxLDL treatment significantly downregulated miR5413p expression levels and upregulated STIM1 expression levels in VSMCs. In addition, oxLDL stimulation enhanced cell viability and migration. The overexpression of miR5413p significantly reversed the oxLDLmediated increase in cell viability and migration, whereas the knockdown of miR5413p expression enhanced the oxLDLmediated effects. STIM1 was confirmed to be a target gene of miR5413p in VSMCs. The knockdown of STIM1 significantly impaired the stimulatory effects of miR5413p knockdown on cell viability and migration. In conclusion, the findings of the present study suggested that miR5413p may efficiently repress VSMC viability and migration by targeting STIM1 under the treatment of oxLDL. These results indicated that the miR5413p/STIM1 axis may represent a potent target to modulate VSMC viability and migration.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
MicroRNAs
/
Atherosclerosis
/
Plaque, Atherosclerotic
/
Stromal Interaction Molecule 1
/
Neoplasm Proteins
Type of study:
Prognostic_studies
Limits:
Humans
Language:
En
Journal:
Mol Med Rep
Year:
2021
Document type:
Article
Country of publication:
Greece