The presence of Prevotella melaninogenica within tissue and preliminary study on its role in the pathogenesis of oral lichen planus.
Oral Dis
; 28(6): 1580-1590, 2022 Sep.
Article
in En
| MEDLINE
| ID: mdl-33780104
ABSTRACT
OBJECTIVE:
Oral lichen planus (OLP) is a chronic inflammatory disease that occurs in the oral mucosa with characteristic white striations lesions, recurrent erosions, and pains. The etiology and pathogenesis of OLP are still unclear. MATERIALS ANDMETHODS:
We analyzed the bacterial community structure of buccal mucosa in patients with OLP and normal controls by high-throughput sequencing. Fluorescence in situ hybridization (FISH) was used to detect Prevotella melaninogenica (P. melaninogenica) in 13 OLP samples and 10 controls. The amounts of P. melaninogenica in OLP buccal mucosa and the expression of inflammatory cytokines in co-culture of mouse-derived macrophages with P. melaninogenica were detected by RT-qPCR.RESULTS:
The P. melaninogenica was more abundant in OLP than in healthy controls, and the differences were significant at the level of the phylum, family, genus, and species (p < .05). FISH showed that P. melaninogenica can invade the epithelium and even the lamina propria of OLP, while no invasion was found in the normal mucosa. Prevotella melaninogenica can adhere to and invade macrophages and then activate the transcription of IL-1ß, IL-6, and TNF-α in NF-κB signaling pathway.CONCLUSION:
Prevotella melaninogenica may be involved in the pathogenic process of OLP, and its specific mechanism deserves further study.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Lichen Planus, Oral
Type of study:
Etiology_studies
Limits:
Animals
Language:
En
Journal:
Oral Dis
Journal subject:
ODONTOLOGIA
Year:
2022
Document type:
Article
Affiliation country:
China