Making the most of high-dimensional cytometry data.
Immunol Cell Biol
; 99(7): 680-696, 2021 08.
Article
in En
| MEDLINE
| ID: mdl-33797774
ABSTRACT
High-dimensional cytometry represents an exciting new era of immunology research, enabling the discovery of new cells and prediction of patient responses to therapy. A plethora of analysis and visualization tools and programs are now available for both new and experienced users; however, the transition from low- to high-dimensional cytometry requires a change in the way users think about experimental design and data analysis. Data from high-dimensional cytometry experiments are often underutilized, because of both the size of the data and the number of possible combinations of markers, as well as to a lack of understanding of the processes required to generate meaningful data. In this article, we explain the concepts behind designing high-dimensional cytometry experiments and provide considerations for new and experienced users to design and carry out high-dimensional experiments to maximize quality data collection.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Flow Cytometry
Limits:
Humans
Language:
En
Journal:
Immunol Cell Biol
Journal subject:
ALERGIA E IMUNOLOGIA
Year:
2021
Document type:
Article
Affiliation country:
Australia