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Comparison of a one-step real-time RT-PCR and a nested real-time RT-PCR for a genogroup II norovirus reveals differences in sensitivity depending upon assay design and visualization.
Manuel, Clyde S; Suther, Cassandra; Moore, Matthew D; Jaykus, Lee-Ann.
Affiliation
  • Manuel CS; Department of Food, Nutrition, and Bioprocessing Sciences, North Carolina State University, Raleigh, NC, United States of America.
  • Suther C; Department of Food Science, University of Massachusetts, Amherst, MA, United States of America.
  • Moore MD; Department of Food, Nutrition, and Bioprocessing Sciences, North Carolina State University, Raleigh, NC, United States of America.
  • Jaykus LA; Department of Food Science, University of Massachusetts, Amherst, MA, United States of America.
PLoS One ; 16(4): e0248581, 2021.
Article in En | MEDLINE | ID: mdl-33831019

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: RNA, Viral / Caliciviridae Infections / Reverse Transcriptase Polymerase Chain Reaction / Norovirus / Feces / Real-Time Polymerase Chain Reaction Type of study: Diagnostic_studies Limits: Female / Humans / Male Language: En Journal: PLoS One Journal subject: CIENCIA / MEDICINA Year: 2021 Document type: Article Affiliation country: United States Country of publication: United States

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: RNA, Viral / Caliciviridae Infections / Reverse Transcriptase Polymerase Chain Reaction / Norovirus / Feces / Real-Time Polymerase Chain Reaction Type of study: Diagnostic_studies Limits: Female / Humans / Male Language: En Journal: PLoS One Journal subject: CIENCIA / MEDICINA Year: 2021 Document type: Article Affiliation country: United States Country of publication: United States