Ca<sup>2+</sup>-activated Cl<sup>-</sup> channel TMEM16A inhibition by cholesterol promotes angiogenesis in endothelial cells.

Ma, Ke; Liu, Sitong; Liang, Hongyue; Wang, Guan; Wang, Tianyu; Luo, Shuya; Gao, Kuan; Wang, Hui; Liu, Mei; Bai, Lichuan; Xiao, Qinghuan

Ca²⁺-activated Cl^- channel TMEM16A inhibition by cholesterol promotes angiogenesis in endothelial cells.

Ma, Ke; Liu, Sitong; Liang, Hongyue; Wang, Guan; Wang, Tianyu; Luo, Shuya; Gao, Kuan; Wang, Hui; Liu, Mei; Bai, Lichuan; Xiao, Qinghuan.

Affiliation

Ma K; Department of Ion Channel Pharmacology, School of Pharmacy, China Medical University, Shenyang 110122, China.
Liu S; Department of Ion Channel Pharmacology, School of Pharmacy, China Medical University, Shenyang 110122, China.
Liang H; Department of Ion Channel Pharmacology, School of Pharmacy, China Medical University, Shenyang 110122, China.
Wang G; Department of Ion Channel Pharmacology, School of Pharmacy, China Medical University, Shenyang 110122, China.
Wang T; Department of Ion Channel Pharmacology, School of Pharmacy, China Medical University, Shenyang 110122, China.
Luo S; Department of Ion Channel Pharmacology, School of Pharmacy, China Medical University, Shenyang 110122, China.
Gao K; Department of Ion Channel Pharmacology, School of Pharmacy, China Medical University, Shenyang 110122, China.
Wang H; Department of Ion Channel Pharmacology, School of Pharmacy, China Medical University, Shenyang 110122, China.
Liu M; Department of Ion Channel Pharmacology, School of Pharmacy, China Medical University, Shenyang 110122, China.
Bai L; Department of Ion Channel Pharmacology, School of Pharmacy, China Medical University, Shenyang 110122, China.
Xiao Q; Department of Ion Channel Pharmacology, School of Pharmacy, China Medical University, Shenyang 110122, China.

J Adv Res ; 29: 23-32, 2021 03.

Article in En | MEDLINE | ID: mdl-33842002

Subject(s)

Anoctamin-1/antagonists & inhibitors; Chloride Channels/metabolism; Cholesterol/pharmacology; Endothelial Cells/drug effects; Neovascularization, Pathologic/metabolism; Anoctamin-1/metabolism; Aorta/metabolism; Blood-Brain Barrier/metabolism; Calcium/metabolism; Cell Movement/drug effects; Cell Proliferation/drug effects; DNA (Cytosine-5-)-Methyltransferase 1/metabolism; Endothelial Cells/metabolism; HEK293 Cells; Humans; Hypertension/metabolism; Patch-Clamp Techniques

Key words

5-aza, 5-Aza-2'-deoxycytidine; ANOVA, analysis of variance; Angiogenesis; CCK-8, Cell Counting Kit-8; CaCCs, Ca2+-activated Cl− currents; Cholesterol; DMEM, Dulbecco's Modified Eagle Medium; DNMT1, DNA methyltransferase 1; EGTA, ethylene glycol-bis(2-aminoethyl ether)-N,N,N',N'-tetraacetic acid; Endothelial cells; FBS, fetal bovine serum; HAECs, human aortic endothelial cells; HEPES, N-2-hydroxyethil-piperazine-N'-2-ethanesulfonic acid; MßCD, methyl-ß cyclodextrin; NMDG, N-methyl-D-glucamine; PVDF, polyvinylidene fluoride; RIPA, radio immunoprecipitation assay; ROS, reactive oxygen species; SE, standard error; TMEM16A; shRNAs, short hairpin RNAs

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Cholesterol / Chloride Channels / Endothelial Cells / Anoctamin-1 / Neovascularization, Pathologic Limits: Humans Language: En Journal: J Adv Res Year: 2021 Document type: Article Affiliation country: China Country of publication: Egypt

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