Quantitation of flurazepam and three metabolites by electron capture gas liquid chromatography.
J Anal Toxicol
; 12(3): 122-5, 1988.
Article
in En
| MEDLINE
| ID: mdl-3386206
ABSTRACT
Flurazepam and three of its metabolic products (desalkyl, hydroxyethyl, and aldehyde metabolites) can be simultaneously quantitated without derivatization by gas chromatography with electron capture detection. After addition of a suitable internal standard, unknown biological samples and calibration standards are extracted at neutral pH into benzene/isoamyl alcohol. The reconstituted extract is chromatographed at 275 degrees C with a 10% OV-101 liquid phase, which allows resolution of all 5 compounds. In some cases a 1% OV-225 liquid phase is used for quantitation of hydroxyethylflurazepam. The method is sufficiently sensitive and reproducible for use in clinical and experimental pharmacokinetic studies.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Flurazepam
Type of study:
Guideline
Limits:
Adult
/
Female
/
Humans
Language:
En
Journal:
J Anal Toxicol
Year:
1988
Document type:
Article