Ecotoxicity testing of airborne particulate matter-comparison of sample preparation techniques for the Vibrio fischeri assay.
Environ Geochem Health
; 43(11): 4367-4378, 2021 Nov.
Article
in En
| MEDLINE
| ID: mdl-33864174
ABSTRACT
The bioassay based on the bioluminescence inhibition of the marine bacterium Vibrio fischeri has been the most widely used test for the assessment of airborne particulate matter ecotoxicity. Most studies available use an extract of the solid sample, either made with water or organic solvents. As an alternative, a whole-aerosol test is also available where test bacteria are in actual contact with contaminated particles. In our study, different extraction procedures were compared to this direct contact test based on the V. fischeri assay and analytical measurements. The lowest PAH content and the highest EC50 were determined in water extract, while the highest PAH amount and lowest EC50 were measured in dichloromethane, hexane, and dimethyl-sulphoxide extracts. EC50 of the direct contact test was comparable to that of the methanol extract. Our results suggest that the sensitivity of the direct contact test equals to that of extraction procedures using organic solvents, moreover, it is mimicking an environmentally realistic exposure route.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Aliivibrio fischeri
/
Particulate Matter
Language:
En
Journal:
Environ Geochem Health
Journal subject:
QUIMICA
/
SAUDE AMBIENTAL
Year:
2021
Document type:
Article
Affiliation country:
Hungary