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Epigenetic effects of insecticides on early differentiation of mouse embryonic stem cells.
Wang, Wenlong; Ito, Tomohiro; Otsuka, Satoshi; Nansai, Hiroko; Abe, Kuniya; Nakao, Yoichi; Ohgane, Jun; Yoneda, Minoru; Sone, Hideko.
Affiliation
  • Wang W; Center for Health and Environmental Risk Research, National Institute for Environmental Studies, 16-2 Onogawa, Tsukuba, Ibaraki 305-8506, Japan; Department of Environmental Engineering, Graduate School of Engineering, Kyoto University, Katsura, Nishikyo-ku, Kyoto 615-8540, Japan.
  • Ito T; Center for Health and Environmental Risk Research, National Institute for Environmental Studies, 16-2 Onogawa, Tsukuba, Ibaraki 305-8506, Japan. Electronic address: itotomo@nies.go.jp.
  • Otsuka S; Center for Health and Environmental Risk Research, National Institute for Environmental Studies, 16-2 Onogawa, Tsukuba, Ibaraki 305-8506, Japan; Graduate School of Advanced Science and Engineering, Waseda University, 3-4-1 Ookubo, Shinjuku-ku, Tokyo 169-8555, Japan.
  • Nansai H; Center for Health and Environmental Risk Research, National Institute for Environmental Studies, 16-2 Onogawa, Tsukuba, Ibaraki 305-8506, Japan.
  • Abe K; Technology and Development Team for Mammalian Genome Dynamics, BioResource Research Center, RIKEN, 3-1-1 Koyadai, Tsukuba, Ibaraki 305-0074, Japan.
  • Nakao Y; Graduate School of Advanced Science and Engineering, Waseda University, 3-4-1 Ookubo, Shinjuku-ku, Tokyo 169-8555, Japan.
  • Ohgane J; Department of Life Sciences, School of Agriculture, Meiji University, 1-1-1 Higashi-mita, Tama-ku, Kawasaki 214-8571, Japan.
  • Yoneda M; Department of Environmental Engineering, Graduate School of Engineering, Kyoto University, Katsura, Nishikyo-ku, Kyoto 615-8540, Japan.
  • Sone H; Center for Health and Environmental Risk Research, National Institute for Environmental Studies, 16-2 Onogawa, Tsukuba, Ibaraki 305-8506, Japan; Department of Environmental Health and Preventive Medicine, Yokohama University of Pharmacy, 601 Matanocho, Totsuka, Yokohama 245-0066, Japan. Electronic a
Toxicol In Vitro ; 75: 105174, 2021 Sep.
Article in En | MEDLINE | ID: mdl-33865946
ABSTRACT
Increasing evidence indicates that many insecticides produce significant epigenetic changes during embryogenesis, leading to developmental toxicities. However, the effects of insecticides on DNA methylation status during early development have not been well studied. We developed a novel nuclear phenotypic approach using mouse embryonic stem cells harboring enhanced green fluorescent protein fused with methyl CpG-binding protein to evaluate global DNA methylation changes via high-content imaging analysis. Exposure to imidacloprid, carbaryl, and o,p'-DDT increased the fluorescent intensity of granules in the nuclei, indicating global DNA methylating effects. However, DNA methylation profiling in cell-cycle-related genes, such as Cdkn2a, Dapk1, Cdh1, Mlh1, Timp3, and Rarb, decreased in imidacloprid treatments, suggesting the potential influence of DNA methylation patterns on cell differentiation. We developed a rapid method for evaluating global DNA methylation and used this approach to show that insecticides pose risks of developmental toxicity through DNA methylation.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: DNA Methylation / High-Throughput Screening Assays / Mouse Embryonic Stem Cells / Insecticides Limits: Animals Language: En Journal: Toxicol In Vitro Journal subject: TOXICOLOGIA Year: 2021 Document type: Article Affiliation country: Japan
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: DNA Methylation / High-Throughput Screening Assays / Mouse Embryonic Stem Cells / Insecticides Limits: Animals Language: En Journal: Toxicol In Vitro Journal subject: TOXICOLOGIA Year: 2021 Document type: Article Affiliation country: Japan