Efficiency of whole-exome sequencing in old world and new world primates using human capture reagents.
J Med Primatol
; 50(3): 176-181, 2021 06.
Article
in En
| MEDLINE
| ID: mdl-33876458
ABSTRACT
BACKGROUND:
Whole-exome sequencing (WES) can expedite research on genetic variation in non-human primate (NHP) models of human diseases. However, NHP-specific reagents for exome capture are not available. This study reports the use of human-specific capture reagents in WES for olive baboons, marmosets, and vervet monkeys.METHODS:
Exome capture was carried out using the SureSelect Human All Exon V6 panel from Agilent Technologies, followed by high-throughput sequencing. Capture of protein-coding genes and detection of single nucleotide variants were evaluated.RESULTS:
Exome capture and sequencing results showed that more than 97% of old world and 93% of new world monkey protein coding genes were detected. Single nucleotide variants were detected across the genomes and missense variants were found in genes associated with human diseases.CONCLUSIONS:
A cost-effective approach based on commercial, human-specific reagents can be used to perform WES for the discovery of genetic variants in these NHP species.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
High-Throughput Nucleotide Sequencing
/
Exome
Limits:
Animals
/
Humans
Language:
En
Journal:
J Med Primatol
Year:
2021
Document type:
Article
Affiliation country:
United States