Taurine suppresses liquid-liquid phase separation of lysozyme protein.
Amino Acids
; 53(5): 745-751, 2021 May.
Article
in En
| MEDLINE
| ID: mdl-33881613
Taurine is a compatible osmolyte that confers stability to proteins. Recent studies have revealed that liquid-liquid phase separation (LLPS) of proteins underlie the formation of membraneless organelles in cells. In the present study, we evaluated the role of taurine on LLPS of hen egg lysozyme. We demonstrated that taurine decreases the turbidity of the polyethylene glycol-induced crowding solution of lysozyme. We also demonstrated that taurine attenuates LLPS-dependent cloudiness of lysozyme solution with 0.5 or 1 M NaCl at a critical temperature. Moreover, we observed that taurine inhibits LLPS formation of a heteroprotein mix solution of lysozyme and ovalbumin. These data indicate that taurine can modulate the formation of LLPS of proteins.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Taurine
/
Muramidase
Limits:
Animals
Language:
En
Journal:
Amino Acids
Journal subject:
BIOQUIMICA
Year:
2021
Document type:
Article
Affiliation country:
Japan
Country of publication:
Austria