High-throughput monitoring of biomass conversion reaction with automatic time-resolved analysis.

Reactions of biomass conversions are of great importance in fine chemistry for substantial development. While numerous studies have been performed to search for functional materials to catalyze biomass conversions, a robust and high-throughput analytical method is rather limited, which may hamper further integration and automation of the reactions. Here we propose an automatic and sequential method for the investigation of glucose conversion. By combining sequential sample injection and high-speed capillary electrophoresis (HSCE) techniques, we can monitor the glucose conversion from the beginning toward the end with a good temporal resolution. The HSCE assays are performed using short capillaries (effective length of 10 cm, i.d./o.d. of 50 µm/365 µm), and the analytes are separated at an electric field of 467 V/cm and are detected by UV-absorption at 200 nm with mixed 0.2 mM CTAB, 10 mM borate, 20 mM sorbic acid (pH 12.2) as the background electrolyte. All compounds involved in the reaction, including all products (fructose, 5-hydroxymethylfurfural, formic acid and levulinic acid) and the remaining substrate glucose, are efficiently separated and simultaneously detected from just one analysis with a temporal resolution of one minute. The method exhibits high-resolution separation, a wide linear range with limit-of-detection down to µg/mL-level, as well as excellent repeatability in sequential analysis. It is indicated that the proposed method is of great value in the analysis of complicated biomass conversion and could be potentially applied in various catalytic chemical reactions.