Signals controlling alternative splicing of major histocompatibility complex H-2 class I pre-mRNA.
Immunogenetics
; 28(2): 81-90, 1988.
Article
in En
| MEDLINE
| ID: mdl-3397132
ABSTRACT
The use of alternative splice acceptor sites during the removal of intron 7 in pre-mRNA splicing produces two forms of H-2Kb protein the predominant form, derived from a transcript that has spliced at the upstream splice acceptor site for exon 8 (long exon 8), and a Kb molecule derived from a transcript that has spliced at the downstream acceptor site for exon 8 (short exon 8). We have identified a potential lariat branch point adenosine for the upstream acceptor splice site. This adenosine is found 28 bp from the splice junction and is contained in the sequence AGTGATGG. D-region genes, which use only the downstream splice site, have the sequence AGTGGTGG. We have used in vitro mutagenesis to change this A of the H-2Kb gene to G and have made the reciprocal change in H-2Dd. Elimination of this adenosine in H-2Kb alters the pattern of pre-mRNA splicing and results in a predominance of the Kb molecules with short exon 8 encoded sequences. However, the addition of an adenosine in H-2Dd is not sufficient to direct splicing to the upstream site.
Search on Google
Collection:
01-internacional
Database:
MEDLINE
Main subject:
RNA, Messenger
/
H-2 Antigens
/
RNA Splicing
Limits:
Animals
Language:
En
Journal:
Immunogenetics
Year:
1988
Document type:
Article