Engineering of a Thermostable Biocatalyst for the Synthesis of 2-O-Glucosylglycerol.

Franceus, Jorick; Ubiparip, Zorica; Beerens, Koen; Desmet, Tom

Franceus, Jorick; Ubiparip, Zorica; Beerens, Koen; Desmet, Tom.

Affiliation

Franceus J; Centre for Synthesis Biology (CSB) Department of Biotechnology, Ghent University, Coupure Links 653, 9000, Gent, Belgium.
Ubiparip Z; Centre for Synthesis Biology (CSB) Department of Biotechnology, Ghent University, Coupure Links 653, 9000, Gent, Belgium.
Beerens K; Centre for Synthesis Biology (CSB) Department of Biotechnology, Ghent University, Coupure Links 653, 9000, Gent, Belgium.
Desmet T; Centre for Synthesis Biology (CSB) Department of Biotechnology, Ghent University, Coupure Links 653, 9000, Gent, Belgium.

Chembiochem ; 22(18): 2777-2782, 2021 09 14.

Article in En | MEDLINE | ID: mdl-33991026

Subject(s)

Bacterial Proteins/metabolism; Glucosides/chemical synthesis; Glucosyltransferases/metabolism; Bacterial Proteins/chemistry; Bacterial Proteins/genetics; Bifidobacterium adolescentis/enzymology; Binding Sites; Biocatalysis; Catalytic Domain; Glucosides/metabolism; Glucosyltransferases/chemistry; Glucosyltransferases/genetics; Kinetics; Leuconostoc mesenteroides/enzymology; Molecular Docking Simulation; Mutagenesis, Site-Directed; Stereoisomerism; Substrate Specificity

Key words

biocatalysis; glucosylglycerol; glycoside phosphorylases; protein engineering; sucrose phosphorylase

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Bacterial Proteins / Glucosides / Glucosyltransferases Type of study: Clinical_trials Language: En Journal: Chembiochem Journal subject: BIOQUIMICA Year: 2021 Document type: Article Affiliation country: Belgium Country of publication: Germany

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