Novel electronic biosensor for automated inoculum preparation to accelerate antimicrobial susceptibility testing.
Sci Rep
; 11(1): 11360, 2021 05 31.
Article
in En
| MEDLINE
| ID: mdl-34059754
ABSTRACT
A key predictor of morbidity and mortality for patients with a bloodstream infection is time to appropriate antimicrobial therapy. Accelerating antimicrobial susceptibility testing from positive blood cultures is therefore key to improving patient outcomes, yet traditional laboratory approaches can require 2-4 days for actionable results. The eQUANT-a novel instrument utilizing electrical biosensors-produces a standardized inoculum equivalent to a 0.5 McFarland directly from positive blood cultures. This proof-of-concept study demonstrates that eQUANT inocula prepared from clinically significant species of Enterobacterales were comparable to 0.5 McF inocula generated from bacterial colonies in both CFU/ml concentration and performance in antimicrobial susceptibility testing, with ≥ 95% essential and categorical agreement for VITEK2 and disk diffusion. The eQUANT, combined with a rapid, direct from positive blood culture identification technique, can allow the clinical laboratory to begin antimicrobial susceptibility testing using a standardized inoculum approximately 2-3 h after a blood culture flags positive. This has the potential to improve clinical practice by accelerating conventional antimicrobial susceptibility testing and the resulting targeted antibiotic therapy.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Microbial Sensitivity Tests
/
Electronics
Type of study:
Diagnostic_studies
/
Evaluation_studies
/
Prognostic_studies
Language:
En
Journal:
Sci Rep
Year:
2021
Document type:
Article
Affiliation country:
United States