Herpes simplex virus co-infection facilitates rolling circle replication of the adeno-associated virus genome.
PLoS Pathog
; 17(6): e1009638, 2021 06.
Article
in En
| MEDLINE
| ID: mdl-34061891
ABSTRACT
Adeno-associated virus (AAV) genome replication only occurs in the presence of a co-infecting helper virus such as adenovirus type 5 (AdV5) or herpes simplex virus type 1 (HSV-1). AdV5-supported replication of the AAV genome has been described to occur in a strand-displacement rolling hairpin replication (RHR) mechanism initiated at the AAV 3' inverted terminal repeat (ITR) end. It has been assumed that the same mechanism applies to HSV-1-supported AAV genome replication. Using Southern analysis and nanopore sequencing as a novel, high-throughput approach to study viral genome replication we demonstrate the formation of double-stranded head-to-tail concatemers of AAV genomes in the presence of HSV-1, thus providing evidence for an unequivocal rolling circle replication (RCR) mechanism. This stands in contrast to the textbook model of AAV genome replication when HSV-1 is the helper virus.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Virus Replication
/
Simplexvirus
/
Dependovirus
/
Coinfection
Type of study:
Risk_factors_studies
Limits:
Animals
/
Humans
Language:
En
Journal:
PLoS Pathog
Year:
2021
Document type:
Article
Affiliation country:
Switzerland