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Make microalgal cultures axenic again - a fast and simple workflow utilizing fluorescence-activated cell sorting.
Doppler, Philipp; Kriechbaum, Ricarda; Singer, Benedikt; Spadiut, Oliver.
Affiliation
  • Doppler P; Research Division Biochemical Engineering, Institute of Chemical, Environmental and Bioscience Engineering, Technische Universität Wien, Gumpendorfer Strasse 1a, 1060 Vienna, Austria. Electronic address: philipp.doppler@tuwien.ac.at.
  • Kriechbaum R; Research Division Biochemical Engineering, Institute of Chemical, Environmental and Bioscience Engineering, Technische Universität Wien, Gumpendorfer Strasse 1a, 1060 Vienna, Austria. Electronic address: ricarda.kriechbaum@tuwien.ac.at.
  • Singer B; Research Division Biochemical Engineering, Institute of Chemical, Environmental and Bioscience Engineering, Technische Universität Wien, Gumpendorfer Strasse 1a, 1060 Vienna, Austria.
  • Spadiut O; Research Division Biochemical Engineering, Institute of Chemical, Environmental and Bioscience Engineering, Technische Universität Wien, Gumpendorfer Strasse 1a, 1060 Vienna, Austria. Electronic address: oliver.spadiut@tuwien.ac.at.
J Microbiol Methods ; 186: 106256, 2021 07.
Article in En | MEDLINE | ID: mdl-34082050
ABSTRACT
Since the removal of contaminations in microalgal cultures is extremely laborious and time-consuming, we developed a rapid workflow to obtain axenicity by a combination of fluorescence-activated cell sorting (FACS) and plate spreading. During method development, several cyanobacteria and green algae strains were successfully made axenic. At the end, method transferability to another FACS device was demonstrated. Our workflow offers great time-savings with less hands-on laboratory work compared to conventional isolation techniques.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Microalgae / Axenic Culture / Flow Cytometry Type of study: Evaluation_studies Language: En Journal: J Microbiol Methods Year: 2021 Document type: Article
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Microalgae / Axenic Culture / Flow Cytometry Type of study: Evaluation_studies Language: En Journal: J Microbiol Methods Year: 2021 Document type: Article