Fusion of Streptococcus iniae α-enolase to IMX313 enhanced antibody titer and survival rate in olive flounder (Paralichthys olivaceus).
Fish Shellfish Immunol
; 115: 70-74, 2021 Aug.
Article
in En
| MEDLINE
| ID: mdl-34089887
ABSTRACT
The polymerization of monomeric antigens can be a strategy to overcome the low immunogenicity of subunit vaccines. IMX313 is a hybrid oligomerization domain of chicken C4bp, and has been demonstrated to have potent activity as adjuvants for the fused antigens in mammals. In the present study, we investigated whether the oligomerization of α-enolase of Streptococcus iniae by fusion with IMX313 affected on antibody induction and on protection against S. iniae infection in olive flounder (Paralichthys olivaceus). The oligomerization of S. iniae enolase by fusion with IMX313 (enolase-IMX313) was verified by non-reducing PAGE, and the antibody titer against enolase in olive flounder immunized with enolase-IMX313 was significantly higher than that in fish immunized with enolase alone. Furthermore, although the survival of olive flounder immunized with enolase alone was low, fish immunized with enolase-IMX313 showed much higher survival (RPS 50%) in accordance with higher serum antibody titer, suggesting that fusion of antigens with IMX313 can be an effective way to enhance protective efficacy of subunit vaccines in olive flounder.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Phosphopyruvate Hydratase
/
Streptococcal Infections
/
Bacterial Proteins
/
Flatfishes
/
Fish Diseases
/
Streptococcus iniae
/
Antibody Formation
Limits:
Animals
Language:
En
Journal:
Fish Shellfish Immunol
Journal subject:
BIOLOGIA
/
MEDICINA VETERINARIA
Year:
2021
Document type:
Article
Affiliation country:
South Korea