Affinity precipitation of monoclonal antibodies using ELP-Z in the elution without resolubilization mode.
J Biotechnol
; 338: 1-4, 2021 Sep 10.
Article
in En
| MEDLINE
| ID: mdl-34197822
ABSTRACT
This paper describes a simplified affinity precipitation process for the purification of mAbs from complex mixtures using elastin-like polypeptide fused to a single Z domain of protein A (ELP-Z). This approach eliminates several steps in the original process by directly extracting the mAb from the affinity precipitate, without the need for resolubilization. The efficacy of this elution without resolubilization (EWR) approach for obtaining pure mAb is demonstrated and the effects of mixing are examined. This simplification of the affinity precipitation process may facilitate the implementation of ELP-Z based mAb bioprocessing, particularly in a continuous scenario.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Antineoplastic Agents, Immunological
/
Antibodies, Monoclonal
Language:
En
Journal:
J Biotechnol
Journal subject:
BIOTECNOLOGIA
Year:
2021
Document type:
Article
Affiliation country:
United States