Your browser doesn't support javascript.
loading
Quantitative phase measurements of human cell nuclei using X-ray ptychography.
Schwenke, Jorg; Yusuf, Mohammed; Shemilt, Laura A; Wagner, Ulrich; Sajid, Atiqa; Morrison, Graeme R; Zhang, Fucai; Parsons, Aaron; Rau, Christoph; Robinson, Ian K.
Affiliation
  • Schwenke J; London Centre for Nanotechnology, University College London, 17-19 Gordon Street, London WC1H 0AH, United Kingdom.
  • Yusuf M; London Centre for Nanotechnology, University College London, 17-19 Gordon Street, London WC1H 0AH, United Kingdom.
  • Shemilt LA; London Centre for Nanotechnology, University College London, 17-19 Gordon Street, London WC1H 0AH, United Kingdom.
  • Wagner U; Diamond Light Source Ltd, Diamond House, Harwell Science and Innovation Campus, Didcot OX11 0DE, United Kingdom.
  • Sajid A; Centre for Regenerative Medicine and Stem Cell Research, Aga Khan University, Karachi, Pakistan.
  • Morrison GR; London Centre for Nanotechnology, University College London, 17-19 Gordon Street, London WC1H 0AH, United Kingdom.
  • Zhang F; London Centre for Nanotechnology, University College London, 17-19 Gordon Street, London WC1H 0AH, United Kingdom.
  • Parsons A; Diamond Light Source Ltd, Diamond House, Harwell Science and Innovation Campus, Didcot OX11 0DE, United Kingdom.
  • Rau C; Diamond Light Source Ltd, Diamond House, Harwell Science and Innovation Campus, Didcot OX11 0DE, United Kingdom.
  • Robinson IK; London Centre for Nanotechnology, University College London, 17-19 Gordon Street, London WC1H 0AH, United Kingdom.
J Synchrotron Radiat ; 28(Pt 4): 1166-1173, 2021 Jul 01.
Article in En | MEDLINE | ID: mdl-34212880
ABSTRACT
The human cell nucleus serves as an important organelle holding the genetic blueprint for life. In this work, X-ray ptychography was applied to assess the masses of human cell nuclei using its unique phase shift information. Measurements were carried out at the I13-1 beamline at the Diamond Light Source that has extremely large transverse coherence properties. The ptychographic diffractive imaging approach allowed imaging of large structures that gave quantitative measurements of the phase shift in 2D projections. In this paper a modified ptychography algorithm that improves the quality of the reconstruction for weak scattering samples is presented. The application of this approach to calculate the mass of several human nuclei is also demonstrated.
Subject(s)
Key words
Fulltext
Add to My VHL
Print
XML
PubMed Links
Search on Google

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Cell Nucleus / Microscopy, Phase-Contrast Limits: Humans Language: En Journal: J Synchrotron Radiat Journal subject: RADIOLOGIA Year: 2021 Document type: Article Affiliation country: United kingdom
Fulltext
Add to My VHL
Print
XML
PubMed Links
Search on Google

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Cell Nucleus / Microscopy, Phase-Contrast Limits: Humans Language: En Journal: J Synchrotron Radiat Journal subject: RADIOLOGIA Year: 2021 Document type: Article Affiliation country: United kingdom