In situ detection of the eIF4F translation initiation complex in mammalian cells and tissues.
STAR Protoc
; 2(3): 100621, 2021 09 17.
Article
in En
| MEDLINE
| ID: mdl-34223196
ABSTRACT
The eukaryotic translation initiation complex eIF4F plays an important role in gene expression. The methods that are used to monitor the formation of the eIF4F complex are usually indirect and provide no information on its subcellular localization. This protocol describes a proximity ligation assay-based procedure allowing the direct in situ visualization of the eIF4F complex, as well as its absolute quantification per cell using adapted image analysis software. For complete details on the use and execution of this protocol, please refer to Boussemart et al. (2014).
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Eukaryotic Initiation Factor-4F
Type of study:
Diagnostic_studies
Limits:
Animals
/
Humans
Language:
En
Journal:
STAR Protoc
Year:
2021
Document type:
Article
Affiliation country:
China