Nandrolone and estradiol biomarkers identification in bovine urine applying a liquid chromatography high-resolution mass spectrometry metabolomics approach.
Drug Test Anal
; 14(5): 879-886, 2022 May.
Article
in En
| MEDLINE
| ID: mdl-34242491
ABSTRACT
With the aim of specifically investigating patterns associated with three steroid treatments (17ß-nandrolone, 17ß-estradiol, and 17ß-nandrolone + 17ß-estradiol) in bovine, an reversed phase liquid chromatography (RPLC)-electrospray ionization (ESI)(+/-)-high-resolution mass spectrometry (HRMS) study was conducted to characterize the urinary profiles of involved animals. Although specific fingerprints with strong differences could be highlighted between urinary metabolite profiles within urine samples collected on control and treated animals, it appeared further that significant discriminations could also be observed between steroid treatments, evidencing thus specific patterns and candidate biomarkers associated to each treatment. An MS-2 structural elucidation step enabled level-1 identification of two biomarkers mainly involved in energy pathways, in relation to skeletal muscle functioning. These results make it possible to envisage a global strategy for the detection of anabolic practices involving steroids, while at the same time providing clues as to the compounds used, which would facilitate the confirmation stage to follow.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Anabolic Agents
/
Nandrolone
Type of study:
Diagnostic_studies
Limits:
Animals
Language:
En
Journal:
Drug Test Anal
Journal subject:
FARMACOLOGIA
Year:
2022
Document type:
Article
Affiliation country:
France