Legumain knockout improves repeated corticosterone injection-induced depression-like emotional and cognitive deficits.
Behav Brain Res
; 413: 113464, 2021 09 10.
Article
in En
| MEDLINE
| ID: mdl-34265317
ABSTRACT
Emotional and cognitive impairment has been recognized as a central feature of depression, which is closely related to hyperfunction of the hypothalamic-pituitary-adrenal (HPA) axis caused by down-regulation of glucocorticoid receptor (GR) expression in patients. A decrease in GR expression can cause pathological changes and lead to the impairment of synaptic plasticity. Legumain, a lysosomal cysteine protease, plays an important role in neurological diseases. It is reported that legumain activates the MAPK signaling pathway, which modifies the GR. Therefore, we hypothesize that regulation of the GR by legumain plays a crucial role in the pathological process of depression. The relationships between legumain, GR, synaptic plasticity and emotional and cognitive deficits were explored in this study. The results demonstrated that repeated corticosterone (CORT) injections (3 weeks) induced emotional and cognitive deficits in mice, based on behavioral experiments and the detection of synaptic plasticity. Furthermore, CORT injections decreased the expression of hippocampal synapse-related proteins, cell density and dendritic spine density in the hippocampus, accompanied by increased protein expression in the MAPK signaling pathway and decreased expression of the GR. In conclusion, our results demonstrated that legumain knockout up-regulated expression of the GR by reducing protein expression in the MAPK signaling pathway, thereby improving hippocampal synaptic plasticity as well as the emotional and cognitive impairment of model mice. This suggests that legumain may be an effective therapeutic target for emotional and cognitive deficits.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Corticosterone
/
Cysteine Endopeptidases
/
MAP Kinase Signaling System
/
Depression
/
Cognitive Dysfunction
/
Neuronal Plasticity
Limits:
Animals
Language:
En
Journal:
Behav Brain Res
Year:
2021
Document type:
Article
Affiliation country:
China