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Evaluation of the eazyplex® EHEC complete (Amplex) assay for the differentiation of stx1 and stx2 positivity in stool samples.
Vigfússon, Hannes Bjarki; Ennefors, Theresa; Kjerstadius, Torbjörn; Sundqvist, Martin.
Affiliation
  • Vigfússon HB; Department of Laboratory Medicine, Clinical Microbiology, Örebro University Hospital, 701 85 Örebro, Sweden. Electronic address: hannes.vigfusson@regionorebrolan.se.
  • Ennefors T; Department of Laboratory Medicine, Clinical Microbiology, Örebro University Hospital, 701 85 Örebro, Sweden.
  • Kjerstadius T; Department of Clinical Microbiology, Central Hospital, 651 85 Karlstad, Sweden.
  • Sundqvist M; Department of Laboratory Medicine, Clinical Microbiology, Örebro University Hospital, 701 85 Örebro, Sweden.
J Microbiol Methods ; 188: 106291, 2021 09.
Article in En | MEDLINE | ID: mdl-34311019
The performance of the eazyplex® EHEC complete (Amplex) for the detection of Shiga toxin genes in stool samples was evaluated. The assay performed well in distinguishing between stx1 and stx2 but suboptimal sensitivity may limit its use to complementary testing rather than primary diagnosis of Shiga toxin-producing Escherichia coli infections.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Shiga Toxin 1 / Shiga Toxin 2 / Molecular Diagnostic Techniques / Escherichia coli Infections / Feces Type of study: Diagnostic_studies Language: En Journal: J Microbiol Methods Year: 2021 Document type: Article Country of publication: Netherlands
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Shiga Toxin 1 / Shiga Toxin 2 / Molecular Diagnostic Techniques / Escherichia coli Infections / Feces Type of study: Diagnostic_studies Language: En Journal: J Microbiol Methods Year: 2021 Document type: Article Country of publication: Netherlands